Right, I bet these biopsies were in fine mesh/holes tissue capsules and air
has little or no escape, so tissues or parts of tissues were not in all
solutions during fixing/ processing!
----- Original Message -----
From: "Rene J Buesa" <rjbu...@yahoo.com>
To: "Cheryl" <tkngfl...@yahoo.com>; <histonet@lists.utsouthwestern.edu>
Sent: Tuesday, December 04, 2012 1:26 PM
Subject: Re: [Histonet] Trichrome
IF you are not successful when the slides are stained on the Nexus OR
manually, while the controls stain well, you can eliminate the instrument as
the cause.
It is most likely a processing artifact: either incomplete fixation, or
overdehydration, or incomplete dewaxing.
Look to your protocol instead to your instrument.
René J.
From: Cheryl <tkngfl...@yahoo.com>
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, December 4, 2012 8:18 AM
Subject: [Histonet] Trichrome
Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers
is inconsistent. Taking it off the stainer and doing it by hand with a kit
has similar results.
The tissue is NBF fixed needle biopsies on kidney. There is a little
mechanical damage on the edges of the tissue but the collagens are staining
too light and the red overstains. The normal tissue as control is
beautiful -- I know I know--don't force the stain--but it doesn't make sense
to have this much variation from a kit or auto stainer...
Help?
Cheryl Kerry, HT(ASCP)
Path Group of Louisiana
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