Michael, we used to cut human brains on a room temp water bath at a hospital I worked at many years ago. It might work for mouse brains too.
Cassie Davis ----- Original Message ----- From: "Michael J. Lyon, Ph.D." <ly...@upstate.edu> To: "Histonet" <histonet@lists.utsouthwestern.edu> Sent: Thursday, January 17, 2013 10:43:02 AM Subject: [Histonet] Paraffin sections mouse brain Hi everyone: While I have sectioned a lot of different stuff, I have not done mouse brain before and I am having considerable difficulty. ??The brains are fixed and were embedded by our pathology department on their automated system. ??When cutting, the paraffin cuts nicely but the brain tissue shreds as if the infiltration was insufficient. ??I have re-embedded one, just melted and new paraffin then vacuum oven for 2 hours. ??The results were the same. ??I don't know if it would help to re-embed going back to EtOH. ??Also, from my distant past I recall that when we were paraffin sectioning whole human larynges, that the face of the block was soaked by placing a wet gauze pad on it. ??I know this help but don't remember what the solution was on the pad. ??Any help would be appreciated. ?? Thanks ?? Michael J. Lyon, PhD Otolaryngology Research Labs SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 ?? Voice: 315-464-7253 Fax: ?? ?? 315-464-5572 ?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 17 Jan 2013 11:21:40 -0600 From: Rita <1949...@gmail.com> Subject: [Histonet] Cpt code 88305 To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <4140c032-e482-45e2-a755-1545d0a66...@gmail.com> Content-Type: text/plain; charset=us-ascii Sorry wrong code it is 88305 I need to know where to get information Thanks Rita Sent from my iPhone ------------------------------ Message: 13 Date: Thu, 17 Jan 2013 11:52:17 -0600 From: Glen Dawson <ihcman2...@hotmail.com> Subject: [Histonet] MOHs Training To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <blu172-w30e142b8158a1b57093ffecc...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" From: ihcman2...@hotmail.com To: histonet-requ...@lists.utsouthwestern.edu Subject: FW: MOHs Training Date: Thu, 17 Jan 2013 07:18:28 -0600 All, My lab is in the process of aiding one of our hospital's sites with MOHs and I'd like to find out what a MOHs lab might charge for training our techs if there was such a service available? What might that process be? How long should it take to get a good histotech with no MOHs experience up and running? What do other labs' training processes look like compared to ours? Is there any place that trains outside techs for such a circumstance? Do you have national contacts that may know someone who knows someone, etc.? Any answers to any/all of the questions above would be an enormous help and would be greatly appreciated. Thank-you in Advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Mercy Health System Janesville, WI ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 110, Issue 23 ***************************************** Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet