The differences might be due to decalcification and/or differences in fixation. I presume you are decalcifying the fish? Are you using EDTA or a formic acid decalcifier? Are they fixed in 10%NBF (or equivalent) for roughly the same amount of time as your human samples? Or are you using a different fixative altogether. The protocol might need to be re-optimized for your fish samples based on those issues.
Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
