Good point by Teri Johnson. I don't decalcify, thus my results may well be compromised by this procedure....and, sure, the bone is disrupted. I am only interested in CNS/PNS so, I don't worry about bone/cartilage disruption. I fix in std 10% NBF. Imho, it is better to "overfix" rather than to worry about optimal short fixation, if you are using HIER.
Utilitarily, Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
