Jack,
That sounds really awesome.

I did some work with the teeth of sows (female pigs) from
specimens collected at slaughter.  Those are very difficult to decalcify,
and when finished, are likely to have no nuclear detail remaining.

Interested to hear what you learn

Wayne Johnson
Beijing Enable Ag Consulting
Yuanmingyuan West Road Meiyuan Com,


On 3:59, Jack Ratliff wrote:
Mes,

This is a very good question and I look forward to answers from individuals 
that have accomplished this with PMMA and a rotary microtome with 
tungsten-carbide knives. If you are talking about an undecalcified specimen 
embedded in PMMA, then I would imagine that the age of the rat could affect the 
ability to achieve an adequate infiltration of the resin. Again, I look forward 
to what others have to say about their success by the method you have outlined.

On the other hand, I know you can achieve the micron thickness you desire if 
you were to use a non-contact femtosecond laser! The machine I am talking about 
is basically a laser microtome manufactured by Rowiak in Germany and it is 
officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany 
has accomplished what you ask with human teeth, revealing the full anatomy of 
the tooth and even with ameloblasts on the enamel surface!

I would be interested to hear more about your project. I will be traveling to 
Germany one week from today to work with this laser microtome until the end of 
the month so I could arrange to have laser cut sections made for you if you are 
interested and unable to make your cuts using PMMA and a rotary microtome. If 
you would like more information, please feel free to contact me by email reply.

Best Regards,

Jack



Jack L Ratliff
Owner/Histologist, Ratliff Histology Consultants, LLC
Chairman, Hard Tissue Committee - National Society for Histotechnology



On Mar 1, 2013, at 7:03 PM, mesruh turkekul<turke...@gmail.com>  wrote:

Dear Histonetters,


I have one more question. Is it possible to obtain 5-10um thick sections of
PMMA embedded teeth using regular Leica paraffin microtome (RM2265)
equipped with disposable tungsten carbide blade?

Thanks,
Mes
On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul<turke...@gmail.com>  wrote:

Dear Histonetters,


I am studying bone and teeth growth in rat maxilla. I will inject calcein
green and would like to fix, embed and sections the rat maxilla.
Any suggestions for the best method to fix, embed and section the samples
for fluorescnet microscopy?

Thank you very much!

Mes HTL (ASCP)
Memorial Sloan-Kettering Cancer Center

On Fri, Mar 1, 2013 at 1:01 PM,<
histonet-requ...@lists.utsouthwestern.edu>  wrote:

Send Histonet mailing list submissions to
        histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
        histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
        histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. FNA Clia Guidelines (PicheGrocki, Jessica)
   2. RE: FNA Clia Guidelines (Horn, Hazel V)
   3. GSH Symposium April 12-14 (Zimmerman, Billie)
   4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC)
   5. (no subject) (Vikrant Piprode)
   6. The GSH 4oth Anniversary Meeting (David Kemler)
   7. QIHC (Renee H. Workman)


----------------------------------------------------------------------

Message: 1
Date: Thu, 28 Feb 2013 20:26:30 +0000
From: "PicheGrocki, Jessica"<jpiche-gro...@wtbyhosp.org>
Subject: [Histonet] FNA Clia Guidelines
To: "histonet@lists.utsouthwestern.edu"
        <histonet@lists.utsouthwestern.edu>
Message-ID:
        <
631955447a364b45b9458d2905635110655d2...@win08-mbx-01.wtbyhosp.org>
Content-Type: text/plain; charset=s-ascii"

Hi All,

Quick question............what are the Clia requirements for Fine needle
aspirate procedures? Is it considered high complexity testing? And who
prepares the slides when the needle is handed off?

Thank you,

Jessica Piche, HT(ASCP)



CONFIDENTIALITY NOTICE: This email and any attachments contain
confidential information that is legally privileged. This information is
intended only for the use of the individual or entity named above. The
authorized recipient of this information is prohibited from disclosing this
information to any other party unless required to do so by law or
regulation. If you are not the intended recipient, you are hereby notified
that any disclosure, copying, distribution or action taken in reliance on
the contents of these documents is strictly prohibited. If you have
received this information in error, please notify the sender immediately
and delete these documents. Copyright (c) Waterbury Hospital


------------------------------

Message: 2
Date: Thu, 28 Feb 2013 15:05:35 -0600
From: "Horn, Hazel V"<hor...@archildrens.org>
Subject: [Histonet] RE: FNA Clia Guidelines
To: "'PicheGrocki, Jessica'"<jpiche-gro...@wtbyhosp.org>,
        "histonet@lists.utsouthwestern.edu"
        <histonet@lists.utsouthwestern.edu>
Message-ID:
        <25a4de08332b19499904459f00aaacb719be135...@evs1.archildrens.org>
Content-Type: text/plain; charset=s-ascii"

Our pathologists do it all.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu [mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of PicheGrocki,
Jessica
Sent: Thursday, February 28, 2013 2:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FNA Clia Guidelines

Hi All,

Quick question............what are the Clia requirements for Fine needle
aspirate procedures? Is it considered high complexity testing? And who
prepares the slides when the needle is handed off?

Thank you,

Jessica Piche, HT(ASCP)



CONFIDENTIALITY NOTICE: This email and any attachments contain
confidential information that is legally privileged. This information is
intended only for the use of the individual or entity named above. The
authorized recipient of this information is prohibited from disclosing this
information to any other party unless required to do so by law or
regulation. If you are not the intended recipient, you are hereby notified
that any disclosure, copying, distribution or action taken in reliance on
the contents of these documents is strictly prohibited. If you have
received this information in error, please notify the sender immediately
and delete these documents. Copyright (c) Waterbury Hospital
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and
confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering
this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please
notify
us immediately by replying to the message and deleting it from your
computer.
Thank you.



------------------------------

Message: 3
Date: Thu, 28 Feb 2013 21:41:34 +0000
From: "Zimmerman, Billie"<bzimm...@gru.edu>
Subject: [Histonet] GSH Symposium April 12-14
To: "histonet@lists.utsouthwestern.edu"
        <histonet@lists.utsouthwestern.edu>
Message-ID:
        <
7b3deb32e69c034eacb479059c5de3ff758...@ex-mlb-03.ad.georgiahealth.edu>

Content-Type: text/plain; charset=s-ascii"

Don't forget to register for the Georgia Society of Histotechnology
meeting April 12-14.
What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us
for education,
networking, and some great scenery!

Wanda Simons
President of GSH

/bz
Augusta State University and Georgia Health Sciences University have
consolidated to become Georgia Regents University. Effective January 9,
2013, my email address has changed to bzimm...@gru.edu. Please update
your address book to reflect this change.


------------------------------

Message: 4
Date: Thu, 28 Feb 2013 20:51:06 -0500
From: "Jerry Santiago, MSEd, HTL (ASCP) QIHC"
        <jerry.santi...@bellsouth.net>
Subject: [Histonet] FSH abstracts deadline
To: histonet@lists.utsouthwestern.edu
Message-ID:<mmcl8cvpbx1vjcacjbxpkjhs.1362102666...@email.android.com>
Content-Type: text/plain; charset=f-8


Dear histonetters,

March 1st is the ddeadline to submit any abstracts consideration for the
2013 Florida Society for Histotechnology meeting. To submit an abstract go
to www.fshgroup.org

Or email to f...@fshgroup.org

Jerry Santiago
FSH

------------------------------

Message: 5
Date: Fri, 1 Mar 2013 00:31:11 -0800 (PST)
From: Vikrant Piprode<vikk_0...@yahoo.com>
Subject: [Histonet] (no subject)
To: "histonet@lists.utsouthwestern.edu"
        <histonet@lists.utsouthwestern.edu>
Message-ID:
        <1362126671.40509.yahoomail...@web162904.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=o-8859-1

hello,
I have tried staining my calvarial bone sections for Tartrate Resistant
Acid Phosphatase but it didnt work out. Can u help me out in this regard.

Thank you
Vikrant Piprode

------------------------------

Message: 6
Date: Fri, 1 Mar 2013 07:42:31 -0800 (PST)
From: David Kemler<histot...@yahoo.com>
Subject: [Histonet] The GSH 4oth Anniversary Meeting
To: Fellow HistoNetters<Histonet@Lists.UTSouthwestern.edu>
Message-ID:
        <1362152551.97608.yahoomail...@web121501.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=o-8859-1

Hi Folks - Just a quick reminder that HistoTALK http://www.histotalk.com/has 
been invited by the GSH board to participate in the festivities again
this year! The GSH meeting is always a great opportunity to help fulfill
your CEU requirements attending workshops by the very best speakers and
presenters in the histology field!

I'm not presenting this year at the GSH, but I will be there with the
HistoTALK "mobile" studio for guest interviews.

Jekyll Island is a fantastic place for a state meeting and a great
opportunity to mix and mingle. Be sure to join us for a way-cool time! GSH
members are FUN folks! I know once you go to just one of their
state meetings - you'll be hooked!

The date is April 12th - 14th, so there's still time to register.

And again, thanks GSH board for inviting me and HistoTALK to be part
of your 40th Anniversary Celebration!

Yours,
David
http://www.histotalk.com/

------------------------------

Message: 7
Date: Fri, 1 Mar 2013 16:26:19 +0000
From: "Renee H. Workman"<rhwork...@uro.com>
Subject: [Histonet] QIHC
To: "histonet@lists.utsouthwestern.edu"
        <histonet@lists.utsouthwestern.edu>
Message-ID:
        <
c9b2d9dc1cb4bf4caaecb401f244490124f1d...@bluprd0511mb413.namprd05.prod.outlook.com

Content-Type: text/plain; charset=so-8859-1"

I will be taking the QIHC soon has anyone taken it recently.  I lost my
QIHC when I changed jobs and want to get

it back.


Renee H. Workman
W: 804-527-1316 | F: 804-270-0917
rhwork...@uro.com<mailto:rhwork...@uro.com>  | www.uro.com<
http://www.uro.com/>





Disclaimer: The email and files transmitted with it are confidential and
are intended solely for the use of the individual or entity to whom they
are addressed. If you are not the original recipient or the person
responsible for the delivering the email to the intended recipient, be
advised that you have received this email in error, and that any use,
dissemination, forwarding, printing or copying of this email is strictly
prohibited. If you received this email in error, please delete it from your
system without copying it, and notify the sender by reply email so that our
address record can be corrected.



------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 112, Issue 1
****************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Reply via email to