Thanks for your message Wayne. I will definitely follow up with you upon my return! Please let me know if there is anything else that interest you with regards to Hard Tissue specimen types. I specifically work with the histology related to bone, biomaterials and medical device implants. In fact, I will be presenting on these topics at several histology meetings here in the U.S. throughout the year:
Indiana Society for Histotechnology - Indianapolis, IN (March 8-9) - "Technological Advancements in Microtomy: A Non-Contact Alternative to Conventional Histology Equipment Techniques" Georgia Society for Histotechnology - Jekyll Island, GA (April 12-13) - "Laser Microtomy: The Future of Soft and Hard Tissue Histology" LINK: http://www.histosearch.com/gsh/symposium.html Polysciences, Inc. Histological Applications and Techniques for Bone, Biomaterials and Medical Device Implants - Cambridge, MA (May 4) - "Acrylic Resins: A Practical Approach for Demonstrating Bone, Biomaterials and Medical Device Implants" LINK: http://www.polysciences.com/Interactive-Histology-Forum-Agenda/187/ Missouri Society for Histotechnology - Columbia, MO (May 30 - June 1) - "Technological Advancements in Microtomy: A Non-Contact Alternative to Conventional Histology Equipment Techniques" LINK: http://www.nsh.org/content/missouri-society-histotechnology-msh National Society for Histotechnology Symposium/Convention - Providence, RI (Sept 20-25) - "A Detailed Examination of Working with Decalcified and Undecalcified Bone in Support of Preclinical and Clinical Research" (co-presenter w/ Robert Skinner) LINK: http://www.histoconvention.org Hopefully I will get to meet you at one of these upcoming meetings! Best Regards, Jack > Date: Mon, 4 Mar 2013 19:47:35 +0800 > From: e...@pigsqq.org > To: ratliffj...@hotmail.com > CC: turke...@gmail.com; histonet@lists.utsouthwestern.edu; > jratl...@ratliffhistology.com > Subject: Re: Re: [Histonet] teeth sectioning > > Jack, > That sounds really awesome. > > I did some work with the teeth of sows (female pigs) from > specimens collected at slaughter. Those are very difficult to decalcify, > and when finished, are likely to have no nuclear detail remaining. > > Interested to hear what you learn > > Wayne Johnson > Beijing Enable Ag Consulting > Yuanmingyuan West Road Meiyuan Com, > > > On 3:59, Jack Ratliff wrote: > > Mes, > > > > This is a very good question and I look forward to answers from individuals > > that have accomplished this with PMMA and a rotary microtome with > > tungsten-carbide knives. If you are talking about an undecalcified specimen > > embedded in PMMA, then I would imagine that the age of the rat could affect > > the ability to achieve an adequate infiltration of the resin. Again, I look > > forward to what others have to say about their success by the method you > > have outlined. > > > > On the other hand, I know you can achieve the micron thickness you desire > > if you were to use a non-contact femtosecond laser! The machine I am > > talking about is basically a laser microtome manufactured by Rowiak in > > Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko > > Richter from Germany has accomplished what you ask with human teeth, > > revealing the full anatomy of the tooth and even with ameloblasts on the > > enamel surface! > > > > I would be interested to hear more about your project. I will be traveling > > to Germany one week from today to work with this laser microtome until the > > end of the month so I could arrange to have laser cut sections made for you > > if you are interested and unable to make your cuts using PMMA and a rotary > > microtome. If you would like more information, please feel free to contact > > me by email reply. > > > > Best Regards, > > > > Jack > > > > > > > > Jack L Ratliff > > Owner/Histologist, Ratliff Histology Consultants, LLC > > Chairman, Hard Tissue Committee - National Society for Histotechnology > > > > > > > > On Mar 1, 2013, at 7:03 PM, mesruh turkekul<turke...@gmail.com> wrote: > > > > > >> Dear Histonetters, > >> > >> > >> I have one more question. Is it possible to obtain 5-10um thick sections of > >> PMMA embedded teeth using regular Leica paraffin microtome (RM2265) > >> equipped with disposable tungsten carbide blade? > >> > >> Thanks, > >> Mes > >> On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul<turke...@gmail.com> wrote: > >> > >> > >>> Dear Histonetters, > >>> > >>> > >>> I am studying bone and teeth growth in rat maxilla. I will inject calcein > >>> green and would like to fix, embed and sections the rat maxilla. > >>> Any suggestions for the best method to fix, embed and section the samples > >>> for fluorescnet microscopy? > >>> > >>> Thank you very much! > >>> > >>> Mes HTL (ASCP) > >>> Memorial Sloan-Kettering Cancer Center > >>> > >>> On Fri, Mar 1, 2013 at 1:01 PM,< > >>> histonet-requ...@lists.utsouthwestern.edu> wrote: > >>> > >>> > >>>> Send Histonet mailing list submissions to > >>>> histonet@lists.utsouthwestern.edu > >>>> > >>>> To subscribe or unsubscribe via the World Wide Web, visit > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> or, via email, send a message with subject or body 'help' to > >>>> histonet-requ...@lists.utsouthwestern.edu > >>>> > >>>> You can reach the person managing the list at > >>>> histonet-ow...@lists.utsouthwestern.edu > >>>> > >>>> When replying, please edit your Subject line so it is more specific > >>>> than "Re: Contents of Histonet digest..." > >>>> > >>>> > >>>> Today's Topics: > >>>> > >>>> 1. FNA Clia Guidelines (PicheGrocki, Jessica) > >>>> 2. RE: FNA Clia Guidelines (Horn, Hazel V) > >>>> 3. GSH Symposium April 12-14 (Zimmerman, Billie) > >>>> 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) > >>>> 5. (no subject) (Vikrant Piprode) > >>>> 6. The GSH 4oth Anniversary Meeting (David Kemler) > >>>> 7. QIHC (Renee H. Workman) > >>>> > >>>> > >>>> ---------------------------------------------------------------------- > >>>> > >>>> Message: 1 > >>>> Date: Thu, 28 Feb 2013 20:26:30 +0000 > >>>> From: "PicheGrocki, Jessica"<jpiche-gro...@wtbyhosp.org> > >>>> Subject: [Histonet] FNA Clia Guidelines > >>>> To: "histonet@lists.utsouthwestern.edu" > >>>> <histonet@lists.utsouthwestern.edu> > >>>> Message-ID: > >>>> < > >>>> 631955447a364b45b9458d2905635110655d2...@win08-mbx-01.wtbyhosp.org> > >>>> Content-Type: text/plain; charset=s-ascii" > >>>> > >>>> Hi All, > >>>> > >>>> Quick question............what are the Clia requirements for Fine needle > >>>> aspirate procedures? Is it considered high complexity testing? And who > >>>> prepares the slides when the needle is handed off? > >>>> > >>>> Thank you, > >>>> > >>>> Jessica Piche, HT(ASCP) > >>>> > >>>> > >>>> > >>>> CONFIDENTIALITY NOTICE: This email and any attachments contain > >>>> confidential information that is legally privileged. This information is > >>>> intended only for the use of the individual or entity named above. The > >>>> authorized recipient of this information is prohibited from disclosing > >>>> this > >>>> information to any other party unless required to do so by law or > >>>> regulation. If you are not the intended recipient, you are hereby > >>>> notified > >>>> that any disclosure, copying, distribution or action taken in reliance on > >>>> the contents of these documents is strictly prohibited. If you have > >>>> received this information in error, please notify the sender immediately > >>>> and delete these documents. Copyright (c) Waterbury Hospital > >>>> > >>>> > >>>> ------------------------------ > >>>> > >>>> Message: 2 > >>>> Date: Thu, 28 Feb 2013 15:05:35 -0600 > >>>> From: "Horn, Hazel V"<hor...@archildrens.org> > >>>> Subject: [Histonet] RE: FNA Clia Guidelines > >>>> To: "'PicheGrocki, Jessica'"<jpiche-gro...@wtbyhosp.org>, > >>>> "histonet@lists.utsouthwestern.edu" > >>>> <histonet@lists.utsouthwestern.edu> > >>>> Message-ID: > >>>> <25a4de08332b19499904459f00aaacb719be135...@evs1.archildrens.org> > >>>> Content-Type: text/plain; charset=s-ascii" > >>>> > >>>> Our pathologists do it all. > >>>> > >>>> Hazel Horn > >>>> Supervisor of Histology/Autopsy/Transcription > >>>> Anatomic Pathology > >>>> Arkansas Children's Hospital > >>>> 1 Children's Way | Slot 820| Little Rock, AR 72202 > >>>> 501.364.4240 direct | 501.364.1241 fax > >>>> hor...@archildrens.org > >>>> archildrens.org > >>>> > >>>> > >>>> > >>>> > >>>> > >>>> > >>>> -----Original Message----- > >>>> From: histonet-boun...@lists.utsouthwestern.edu [mailto: > >>>> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, > >>>> Jessica > >>>> Sent: Thursday, February 28, 2013 2:27 PM > >>>> To: histonet@lists.utsouthwestern.edu > >>>> Subject: [Histonet] FNA Clia Guidelines > >>>> > >>>> Hi All, > >>>> > >>>> Quick question............what are the Clia requirements for Fine needle > >>>> aspirate procedures? Is it considered high complexity testing? And who > >>>> prepares the slides when the needle is handed off? > >>>> > >>>> Thank you, > >>>> > >>>> Jessica Piche, HT(ASCP) > >>>> > >>>> > >>>> > >>>> CONFIDENTIALITY NOTICE: This email and any attachments contain > >>>> confidential information that is legally privileged. This information is > >>>> intended only for the use of the individual or entity named above. The > >>>> authorized recipient of this information is prohibited from disclosing > >>>> this > >>>> information to any other party unless required to do so by law or > >>>> regulation. If you are not the intended recipient, you are hereby > >>>> notified > >>>> that any disclosure, copying, distribution or action taken in reliance on > >>>> the contents of these documents is strictly prohibited. If you have > >>>> received this information in error, please notify the sender immediately > >>>> and delete these documents. Copyright (c) Waterbury Hospital > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>>> > >>>> ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > >>>> The information contained in this message may be privileged and > >>>> confidential > >>>> and protected from disclosure. If the reader of this message is not the > >>>> intended recipient, or an employee or agent responsible for delivering > >>>> this > >>>> message to the intended recipient, you are hereby notified that any > >>>> dissemination, distribution or copying of this communication is strictly > >>>> prohibited. If you have received this communication in error, please > >>>> notify > >>>> us immediately by replying to the message and deleting it from your > >>>> computer. > >>>> Thank you. > >>>> > >>>> > >>>> > >>>> ------------------------------ > >>>> > >>>> Message: 3 > >>>> Date: Thu, 28 Feb 2013 21:41:34 +0000 > >>>> From: "Zimmerman, Billie"<bzimm...@gru.edu> > >>>> Subject: [Histonet] GSH Symposium April 12-14 > >>>> To: "histonet@lists.utsouthwestern.edu" > >>>> <histonet@lists.utsouthwestern.edu> > >>>> Message-ID: > >>>> < > >>>> 7b3deb32e69c034eacb479059c5de3ff758...@ex-mlb-03.ad.georgiahealth.edu> > >>>> > >>>> Content-Type: text/plain; charset=s-ascii" > >>>> > >>>> Don't forget to register for the Georgia Society of Histotechnology > >>>> meeting April 12-14. > >>>> What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us > >>>> for education, > >>>> networking, and some great scenery! > >>>> > >>>> Wanda Simons > >>>> President of GSH > >>>> > >>>> /bz > >>>> Augusta State University and Georgia Health Sciences University have > >>>> consolidated to become Georgia Regents University. Effective January 9, > >>>> 2013, my email address has changed to bzimm...@gru.edu. Please update > >>>> your address book to reflect this change. > >>>> > >>>> > >>>> ------------------------------ > >>>> > >>>> Message: 4 > >>>> Date: Thu, 28 Feb 2013 20:51:06 -0500 > >>>> From: "Jerry Santiago, MSEd, HTL (ASCP) QIHC" > >>>> <jerry.santi...@bellsouth.net> > >>>> Subject: [Histonet] FSH abstracts deadline > >>>> To: histonet@lists.utsouthwestern.edu > >>>> Message-ID:<mmcl8cvpbx1vjcacjbxpkjhs.1362102666...@email.android.com> > >>>> Content-Type: text/plain; charset=f-8 > >>>> > >>>> > >>>> Dear histonetters, > >>>> > >>>> March 1st is the ddeadline to submit any abstracts consideration for the > >>>> 2013 Florida Society for Histotechnology meeting. To submit an abstract > >>>> go > >>>> to www.fshgroup.org > >>>> > >>>> Or email to f...@fshgroup.org > >>>> > >>>> Jerry Santiago > >>>> FSH > >>>> > >>>> ------------------------------ > >>>> > >>>> Message: 5 > >>>> Date: Fri, 1 Mar 2013 00:31:11 -0800 (PST) > >>>> From: Vikrant Piprode<vikk_0...@yahoo.com> > >>>> Subject: [Histonet] (no subject) > >>>> To: "histonet@lists.utsouthwestern.edu" > >>>> <histonet@lists.utsouthwestern.edu> > >>>> Message-ID: > >>>> <1362126671.40509.yahoomail...@web162904.mail.bf1.yahoo.com> > >>>> Content-Type: text/plain; charset=o-8859-1 > >>>> > >>>> hello, > >>>> I have tried staining my calvarial bone sections for Tartrate Resistant > >>>> Acid Phosphatase but it didnt work out. Can u help me out in this regard. > >>>> > >>>> Thank you > >>>> Vikrant Piprode > >>>> > >>>> ------------------------------ > >>>> > >>>> Message: 6 > >>>> Date: Fri, 1 Mar 2013 07:42:31 -0800 (PST) > >>>> From: David Kemler<histot...@yahoo.com> > >>>> Subject: [Histonet] The GSH 4oth Anniversary Meeting > >>>> To: Fellow HistoNetters<Histonet@Lists.UTSouthwestern.edu> > >>>> Message-ID: > >>>> <1362152551.97608.yahoomail...@web121501.mail.ne1.yahoo.com> > >>>> Content-Type: text/plain; charset=o-8859-1 > >>>> > >>>> Hi Folks - Just a quick reminder that HistoTALK > >>>> http://www.histotalk.com/has been invited by the GSH board to > >>>> participate in the festivities again > >>>> this year! The GSH meeting is always a great opportunity to help fulfill > >>>> your CEU requirements attending workshops by the very best speakers and > >>>> presenters in the histology field! > >>>> > >>>> I'm not presenting this year at the GSH, but I will be there with the > >>>> HistoTALK "mobile" studio for guest interviews. > >>>> > >>>> Jekyll Island is a fantastic place for a state meeting and a great > >>>> opportunity to mix and mingle. Be sure to join us for a way-cool time! > >>>> GSH > >>>> members are FUN folks! I know once you go to just one of their > >>>> state meetings - you'll be hooked! > >>>> > >>>> The date is April 12th - 14th, so there's still time to register. > >>>> > >>>> And again, thanks GSH board for inviting me and HistoTALK to be part > >>>> of your 40th Anniversary Celebration! > >>>> > >>>> Yours, > >>>> David > >>>> http://www.histotalk.com/ > >>>> > >>>> ------------------------------ > >>>> > >>>> Message: 7 > >>>> Date: Fri, 1 Mar 2013 16:26:19 +0000 > >>>> From: "Renee H. Workman"<rhwork...@uro.com> > >>>> Subject: [Histonet] QIHC > >>>> To: "histonet@lists.utsouthwestern.edu" > >>>> <histonet@lists.utsouthwestern.edu> > >>>> Message-ID: > >>>> < > >>>> c9b2d9dc1cb4bf4caaecb401f244490124f1d...@bluprd0511mb413.namprd05.prod.outlook.com > >>>> > >>>> Content-Type: text/plain; charset=so-8859-1" > >>>> > >>>> I will be taking the QIHC soon has anyone taken it recently. I lost my > >>>> QIHC when I changed jobs and want to get > >>>> > >>>> it back. > >>>> > >>>> > >>>> Renee H. Workman > >>>> W: 804-527-1316 | F: 804-270-0917 > >>>> rhwork...@uro.com<mailto:rhwork...@uro.com> | www.uro.com< > >>>> http://www.uro.com/> > >>>> > >>>> > >>>> > >>>> > >>>> > >>>> Disclaimer: The email and files transmitted with it are confidential and > >>>> are intended solely for the use of the individual or entity to whom they > >>>> are addressed. If you are not the original recipient or the person > >>>> responsible for the delivering the email to the intended recipient, be > >>>> advised that you have received this email in error, and that any use, > >>>> dissemination, forwarding, printing or copying of this email is strictly > >>>> prohibited. If you received this email in error, please delete it from > >>>> your > >>>> system without copying it, and notify the sender by reply email so that > >>>> our > >>>> address record can be corrected. > >>>> > >>>> > >>>> > >>>> ------------------------------ > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>>> End of Histonet Digest, Vol 112, Issue 1 > >>>> **************************************** > >>>> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > > > > >
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