Did you use a serum block ahead of your primary antibody ? If yes, what species was it derived from?
In what species was your primary antibody generated ? --- On Thu, 3/7/13, Nicole Cosenza <ncose...@siumed.edu> wrote: From: Nicole Cosenza <ncose...@siumed.edu> Subject: [Histonet] problem with DAB staining To: histonet@lists.utsouthwestern.edu Date: Thursday, March 7, 2013, 11:50 AM Hello all: I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
