The most likey reasons are the tissue dried out at some point or the slide was not adequately rinsed between steps Rena Fail
On Thu, Mar 7, 2013 at 1:39 PM, Rob Geske <rgeske_2...@yahoo.com> wrote: > Did you use a serum block ahead of your primary antibody ? If yes, what > species was it derived from? > > In what species was your primary antibody generated ? > > > > --- On Thu, 3/7/13, Nicole Cosenza <ncose...@siumed.edu> wrote: > > From: Nicole Cosenza <ncose...@siumed.edu> > Subject: [Histonet] problem with DAB staining > To: histonet@lists.utsouthwestern.edu > Date: Thursday, March 7, 2013, 11:50 AM > > Hello all: > > I recently did IHC on paraffin embedded slides via the DAB method. Upon > addition of the DAB, the entire section turned brown. I know this is not > true staining, as I don't expect a high yield of my protein of interest. > Any suggestions as to why this happened and what to do about it? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
