This practice is listed as a QC measure for issues of cross contamination in the ASCP publication "Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides.
Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tnma...@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 14 Mar 2013 17:52:39 +0000 > Subject: [Histonet] RE: AFB and negative control > > While I don't use a negative control for the AFB, I will use distilled water > throughout the procedure. Most of the time the water in the waterbath is > distilled as well, to rule out contamination there as well. Make sure the > waterbath has been disinfected. > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnma...@mdanderson.org > > > > > Date: Thu, 14 Mar 2013 12:42:30 +0000 > From: Ian R Bernard <ibern...@uab.edu> > Subject: [Histonet] AFB and Negative Control > To: Lee & Peggy Wenk <lpw...@sbcglobal.net>, "Tighe, Sean T" > <sti...@ufl.edu>, "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <d4f4c602b10b9f45b4e9271af6380e16181a1...@uabexmb1.ad.uab.edu> > Content-Type: text/plain; charset="utf-8" > > The only special stain that I know that requires the use of a negative > control is for the AFB. I understand to rule out false positives as the AFB > bacteria might exist in tap water. Nevertheless, a good QA practice which we > will implement now. > > Other than Carson, does anyone know of a regulatory or accreditation agency > is requiring this as well? Any suggestion on a good control tissue type? > Carson recommends uterus. Also if there is a pick up on the negative slide > (link to the tap water) will use of distilled water and a repeat procedure > fix this? > > Any thoughts from fellow histonetters? > > Thanks > Ian Bernard > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
