Isn't a gram stain the only special that requires a positive and negative control?
Sent from my iPhone On Mar 14, 2013, at 1:51 PM, joelle weaver <joellewea...@hotmail.com> wrote: > This practice is listed as a QC measure for issues of cross contamination in > the ASCP publication "Quality Management in Anatomic Pathology, Nakhleh, R. > M.D. I have never had any issues that were persistant enough to warrant this > measure myself, but it is one of the suggestions made under the section for > use of control tissue/slides. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: tnma...@mdanderson.org >> To: histonet@lists.utsouthwestern.edu >> Date: Thu, 14 Mar 2013 17:52:39 +0000 >> Subject: [Histonet] RE: AFB and negative control >> >> While I don't use a negative control for the AFB, I will use distilled water >> throughout the procedure. Most of the time the water in the waterbath is >> distilled as well, to rule out contamination there as well. Make sure the >> waterbath has been disinfected. >> >> Toysha N. Mayer, MBA, HT (ASCP) >> Instructor, Education Coordinator >> Program in Histotechnology >> School of Health Professions >> MD Anderson Cancer Center >> (713) 563-3481 >> tnma...@mdanderson.org >> >> >> >> >> Date: Thu, 14 Mar 2013 12:42:30 +0000 >> From: Ian R Bernard <ibern...@uab.edu> >> Subject: [Histonet] AFB and Negative Control >> To: Lee & Peggy Wenk <lpw...@sbcglobal.net>, "Tighe, Sean T" >> <sti...@ufl.edu>, "histonet@lists.utsouthwestern.edu" >> <histonet@lists.utsouthwestern.edu> >> Message-ID: >> <d4f4c602b10b9f45b4e9271af6380e16181a1...@uabexmb1.ad.uab.edu> >> Content-Type: text/plain; charset="utf-8" >> >> The only special stain that I know that requires the use of a negative >> control is for the AFB. I understand to rule out false positives as the AFB >> bacteria might exist in tap water. Nevertheless, a good QA practice which we >> will implement now. >> >> Other than Carson, does anyone know of a regulatory or accreditation agency >> is requiring this as well? Any suggestion on a good control tissue type? >> Carson recommends uterus. Also if there is a pick up on the negative slide >> (link to the tap water) will use of distilled water and a repeat procedure >> fix this? >> >> Any thoughts from fellow histonetters? >> >> Thanks >> Ian Bernard >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
