Hello Paula, At my previous lab at UCSF, we often had a lot of large & small tissue to freeze & therefore we used quite a bit of liquid nitrogen. We would get our LN2 from a local vendo, & was delivered in a low pressure 230 tall slender LN2 tank. The tank had a nozzle attached for easy & safe dispensing. We then dispensed LN2 into 1 and 2 liter Dewar flasks. These flasks were keep at room temp (under the hood), ready to use during freezing.
This 230 liter tank was strapped stored in a corner of the lab (room temp) & the LN2 inside the tank would last about 3 weeks. The LN2 vendor automatically made delivers of LN2 to our lab - once a month & cost under $100/month. This local LN2 vendor, also carries smaller tanks filled with LN2 which can be stored at room temp., with dispensing nozzles. Paula, this is another option for you - but you'd still need to purchase a Dewar flask to use during freezing. Hope all is well. Best Maria Mejia Affymetrix, Inc On Apr 16, 2013, at 10:35 AM, Paula Sicurello wrote: > Hello HistoNetters, > > I have been having a discussion with my boss about whether or not to store > the isopentane (used for freezing muscle biopsies) in the refrigerator. (An > expensive, explosion proof one that I would have to buy.) > > Her only experience is with the isopentane stored in the refrigerator, my > experiences are with storing it at room temperature. > > What do you all do? Room temp or refrigerated? > > Any and all comments are greatly appreciated. > > Thanks! > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet