We stored at RT but lab did not have a flammable storage cabinet . Since we did not have an explosion proof refrigerator or cold room, it was deemed too dangerous to store in those areas.
There is good reason why one doesn't store in regular refrigerators/cold room. Histologic, Vol. XXXVI, No.1, May 2003, Robert Skinner and Dana Gaddy article t"More than Just A Mandatory Excercise" to see the total, costly ($200,000) destruction of a laboratory at University of Arkansas as the result of a loose lid on an isopentane bottle stored in a non-explosion proof freezer. Fortunately this happened in very early morning when no one was in the lab. It is also not good to breathe the isopentane fumes so working inside a hood is mandatory. We eventually discontinued use of isopentane (2 methyl butane) and used a simple method with LN2 cooled petri dish (supported on a platform) in a styrofoam box for snap freezing multiple tissue blocks (as many as 40) in a tissue dissection/collection session. 1. If you have to have it in lab without an explosion proof refrigerator or freezer, store at RT in flammable cabinet. Gayle Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet