dingers...@aplaboratories.com
---= ----- Original Message --------
Subject: [Histonet] RE: Histonet Dige= st, Vol 113, Issue 30
From: "Barr,Kaye H" <[1]khb...@mdanderson.org>
Date: Tue, April 30, = 2013 1:48 pm
To: "'[2]histonet@lists.utsouthwestern.edu'"
<[3]histonet@lists.utsouthwestern.edu>
What title do most use for someone who does gross and is not a P A?
-----Original Message-----
From:= [4]histonet-boun= c...@lists.utsouthwestern.edu
[[5]mailto:histonet-boun...@lists.utsouthwestern.edu] O= n Behalf Of
[6]hi= stonet-requ...@lists.utsouthwestern.edu
Sent: Tuesday, April 30, = 2013 12:02 PM
To: [7]histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol= 113, Issue 30
Send Histonet mailing list submissions to
=
[8]histonet@lists.u= tsouthwestern.edu
To subscribe or unsubscribe via the Worl= d Wide Web, visit
[9]http://lists.utsouthwestern.edu/mailman/listinfo/hist= onet
or, via email, send a message with subject or body 'help' to
[10]hist= onet-requ...@lists.utsouthwestern.edu
You can reach the pe= rson managing the list at
[11]histonet-ow...@lists.utsouthwestern.edu
=
When replying, please edit your Subject line so it is more
specific
=
than "Re: Contents of Histonet digest..."
Today's To= pics:
1. BIG PROBLEM (Behnaz Sohrab)
2. Re: BIG PR= OBLEM (Linda)
3. Yearly Training (Nancy Schmitt)
4. HIER = pressure cookers.... (k. leigh adams)
5. RE: HIER pressure cookers= .... (Connolly, Brett M)
6. RE: HIER pressure cookers.... (Blazek,= Linda)
7. Re: HIER pressure cookers.... (Jan Shivers)
8.= RE: 5 gal specimen container search (Hannen, Valerie)
9. RE: 5 ga= l specimen container search (Shirley A. Powell)
10. RE: HIER pressu= re cookers.... ([12]Dingersoll
@aplaboratories.com)
11. AW: [Histonet] BIG PROBLEM (Gudrun Lan= g)
12. Processor question - alcoholic formalin vs. buffered forma lin
(Lake, Kim S)
13. RE: Processor question - alcoholi= c formalin vs. buffered
formalin (Hannen, Valerie)
14. = cassette labelers (Margiotta-Watz, Michele)
----------- -----------------------------------------------------------
Me= ssage: 1
Date: Mon, 29 Apr 2013 10:36:01 -0700
From: "Behnaz So= hrab" <[13]sohra...@ah.org>
= Subject: [Histonet] BIG PROBLEM
To: <[14]histonet@lists.utsouthwestern.edu>
Mes= sage-ID: <[15]517E4D11.4347.0= 05...@ah.org>
Content-Type: text/plain; charset="us-ascii"
Good Morning, We have two Leica H&E stainer( old xt-5010 an= d new
5020). We do use : Gill's 3 for 6 minutes,
4% Glacial ACETIC Ac= id 40 seconds
Bluing : 45 sec. We done this for years!! last few mont= hs our
Pantologists are complaining about GI Biopsies that they have TOO mu ch
blue in cytoplasma and the Mucin is too purple and blue? Please any
sugg= estion??
Mean while we are trying the NEW kit from Leica for H&E = , they
think that is TOO pale?any of you has tried this new stain from Leci a?
With much application.
Behnaz Sohrab, Los Angeles
------------------------------
Message: 2
Dat= e: Mon, 29 Apr 2013 11:30:38 -0700 (PDT)
From: Linda <[16]lmd...@yahoo.com>
Subject: Re: [Histon= et] BIG PROBLEM
To: Behnaz Sohrab <[17]sohra...@ah.org>,
"[18]histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwester= n.edu>
Message-ID:
<[19]1367260238.82176.YahooMailN e...@web164002.mail.gq1.yahoo.com>
Content-Type: text/plain; cha= rset=iso-8859-1
Hi,
?
I would first check with y= our alcohol and xylene suppliers to see
if they changed anything.? When som= ething?goes south it is usually
because the manufacturer have changed.? Thi= s happened with my silver
stains.
?
You may have to go back to = the basics- harris hematoxylin, acid
alcohol, and eosin y.? Richard Allen 7= 211 hematoxylin and the rest
of their staining is excellent in these situat= ions until you can get
it back under control.? If you send them slides they= will work a time
out for you.
?
If you are using Leica's selec= tech, which is what I started this
lab out with because I was heavily sampl= ed out,?it has blue nuclei
instead of the traditional purple.? I do 5 minut= es in hematoxylin,
30 dips in clarifier and 1 minute in blue, 55 sec in eos= in but I
have (2) 95s after.? They also have a tech dept to help.? I have a
Leica autostainer too.
?
I hope this helps.
?
Lind= a Dee, HT(ASCP)
?
______________________________= __
From: Behnaz Sohrab <[20]Sohrab= b...@ah.org>
To: [21]histonet@lists.utsouthwestern.edu
Sent: Monday, April 29, = 2013 12:36 PM
Subject: [Histonet] BIG PROBLEM
G= ood Morning, We have two Leica H&E stainer( old xt-5010 and new
5020). = We do use : Gill's 3 for 6 minutes,
4% Glacial ACETIC Acid 40 seconds
Bluing : 45 sec. We done this for years!! last few months our
Pantol= ogists are complaining about GI Biopsies that they have TOO
much blue in cy= toplasma and the Mucin is too purple and blue? Please
any suggestion??
=
Mean while we are trying the NEW kit from Leica for H&E , they
think= that is TOO pale?any of you has tried this new stain from
Lecia?
Wit= h much application.
Behnaz Sohrab, Los Angeles
_________= ______________________________________
Histonet mailing list
[22]Histonet@lists.utsouthwe= stern.edu
[23]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
=
Date: Mon, 29 Apr 2013 18:39:17 +0000
From: Nancy Schmitt <nancy_schm...@pa-ucl.com>
Subject: [Histonet] Yearly Training
To: "[24]histonet@lists.utsouthwestern.edu"
= <[25]histonet@lists.= utsouthwestern.edu>
Message-ID:
<[26]906B4DA90E d1db4db6c7e94d7cee6c36813c0...@peitha.wad.pa-ucl.com>
Content-= Type: text/plain; charset="us-ascii"
Cindy-
We also ha= ve a yearly competency packet. It follows JCAHO
standards and includes dir= ect observations, log documentation for QC
and maintenance and problem solv= ing through written exam.
Systems covered are H&E, IHC, Special s= tains,
processing/embedding, Cytology prep, and Misc. Histology.
Nanc= y Schmitt
Histology Coordinator
Dubuque, IA
------= -----------------------------------------------
From: = Cindy Pyse [[27]mailto:cpyse@x-celllab.c= om]
Sent: Friday, April 26, 2013 4:49 PM
To: '[28]histonet-boun...@lists.utso= uthwestern.edu'
Subject: Yearly training
Happy Friday Everyone
I just wanted an opinion on what eve= ryone's facilities train for
annually.
An example is HIPPA training.= I know what my regs require, I just
wanted to see what everyone else in hi= stoland was doing. Thanks in
advance for all responses. I hope everyone has= a great weekend.
Finally we are going to have a good weather weekend in We= stern NY.
Cindy
NOTI= CE: This email may contain legally privileged information.
The information<= br>
is for the use of only the intended
recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify
the s= ender
that you have received it in error and then delete it along wit= h
any
attachments. Thank you.
-----= -------------------------
Message: 4
Date: Tue, 30 Apr 2= 013 09:29:33 -0400
From: "k. leigh adams" <[29]k.leigh.adams....@gmail.com>
Subject: = [Histonet] HIER pressure cookers....
To: [30]histonet@lists.utsouthwestern.edu
Messag= e-ID:
<[31]CAHot8dV4sR0aDAgb9+Ggbd6tH3CbrRbs0TdrB1BU0hob
rhb...@mail.gmail.com>
Content-Type: text/plain; charset=ISO= -8859-1
Any input as to preferred instruments would be greatly
appreciated...
Leigh
--------------------= ----------
Message: 5
Date: Tue, 30 Apr 2013 09:53:50 -0= 400
From: "Connolly, Brett M" <[32]brett_conno...@merck.com>
Subject: RE: [Histonet] HIE= R pressure cookers....
To: "k. leigh adams" <[33]k.leigh.adams....@gmail.com>,
"[34]histonet@lists.utsouthwe= stern.edu"
<[35]histonet@lists.utsouthwestern.edu>
Message-ID:
<[36]C01C35B84DCDCE49BC60867E87F1C8FE68D4403906@USCTMXP51015.merck
.com>
Content-Type: text/plain; charset="us-ascii"
Biocare Decloaker here !!
Brett M. Connolly, Ph.D.
P= rincipal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box = 4, WP-44K
West Point, PA 19486
[37]brett_conno...@merck.com
T- 215-652-2501
F- 215= -993-6803
-----Original Mes= sage-----
From: [38]histonet-boun...@lists.utsouthwestern.edu
[[39]mailto:histonet-boun...@lists.utso= uthwestern.edu] On Behalf Of
k. leigh adams
Sent: Tuesday, April = 30, 2013 9:30 AM
To: [40]histonet@lists.utsouthwestern.edu
Subject: [Histonet] HIER p= ressure cookers....
Any input as to preferred instruments woul= d be greatly
appreciated...
Leigh
______________________= _________________________
Histonet mailing list
[41]Histonet@lists.utsouthwestern.edu
[42]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notic= e: This e-mail message, together with any attachments,
contains
info= rmation of Merck & Co., Inc. (One Merck Drive, Whitehouse
Station,
=
New Jersey, USA 08889), and/or its affiliates Direct contact
information
for affiliates is available at
[43]http://www.merck.com/contact/contacts.html) th= at may be
confidential,
proprietary copyrighted and/or legally privil= eged. It is intended
solely
for the use of the individual or entity n= amed on this message. If
you are
not the intended recipient, and have= received this message in
error,
please notify us immediately by repl= y e-mail and then delete it
from
your system.
------------------------------
Message: 6
Da= te: Tue, 30 Apr 2013 09:58:58 -0400
From: "Blazek, Linda" <[44]lblazek@digestivespecialists.c= om>
Subject: RE: [Histonet] HIER pressure cookers....
To= : "Connolly, Brett M" <[45]brett= _conno...@merck.com>, "k.
leigh adams"
<[46]k.leigh.adams....@gmail.com>,
"histonet@lists.utsouthwester= n.edu"
<[47]histonet@lists.utsouthwestern.edu>
Message-ID:
<= [48]5A2BD13465E061429D6455C8D6B40E391652B8729D@IBMB7E
xchange.digestivespecialists.com>
Content-Type: text= /plain; charset="us-ascii"
I second that!
Linda= Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
=
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lblazek@digestivespecialist= s.com
-----Original Message-----
From:= [49]histonet-boun= c...@lists.utsouthwestern.edu
[[50]mailto:histonet-boun...@lists.utsouthwestern.edu] O= n Behalf Of
Connolly, Brett M
Sent: Tuesday, April 30, 2013 9:54 AM
To: k. leigh adams;
[51]histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HIER= pressure cookers....
Biocare Decloaker here !!
B= rett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merc= k & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
<= a
href="mailto:brett_conno...@merck.com";>brett_conno...@merck.com
=
T- 215-652-2501
F- 215-993-6803
-----Original Message-----
From: [52]histonet-bounces@lists.utsouthwester= n.edu [[53]mai
lto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of k. leigh
ad= ams
Sent: Tuesday, April 30, 2013 9:30 AM
To: [54]histonet@lists.utsouthwestern.edu
Subject: [Histonet]
HIER pressure cookers....
Any input a= s to preferred instruments would be greatly
appreciated...
Lei= gh
_______________________________________________
Histonet mai= ling list
[55]Histon= e...@lists.utsouthwestern.edu
[56]http://lists.utsouthwestern.edu/mailman/li= stinfo/histonet
Notice: This e-mail message, together with any a= ttachments,
contains information of Merck & Co., Inc. (One Merck Drive,
Whitehouse Station, New Jersey, USA 08889), and/or its affiliates
Direct c= ontact information for affiliates is available at
[57]http://www.merck.com/contact/contacts.h= tml) that may be
confidential, proprietary copyrighted and/or legally p= rivileged. It
is intended solely for the use of the individual or entity na= med on
this message. If you are not the intended recipient, and have receiv ed
this message in error, please notify us immediately by reply e-mail
and = then delete it from your system.
______________________= _________________________
Histonet mailing list
[58]Histonet@lists.utsouthwestern.edu
[59]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
=
------------------------------
Message: 7
=
Date: Tue, 30 Apr 2013 09:34:56 -0500
From: Jan Shivers <shive...@umn.edu>
Subject: Re: [= Histonet] HIER pressure cookers....
To: "Blazek, Linda" <[60]lblazek@digestivespecialists.c= om>
Cc: "[61]= histonet@lists.utsouthwestern.edu"
<[62]histonet@lists.utsouthwestern.edu>, = "k. leigh adams"
<[63]k.leigh.adams....@gmail.com>
Message-ID:
<CAEoC1q3tgnQTBbQYuLKzwh2symq+MbYLNf_0PgD18SUo+4+mWg@mail.gmail.
com= >
Content-Type: text/plain; charset=ISO-8859-1
Thir= d that.
Jan S
On Tue, Apr 30= , 2013 at 8:58 AM, Blazek, Linda <
[64]lbla...@digestivespecialists.com> wrote:
> I second that!
>
> Linda Blazek HT (ASCP)
=
> Manager/Supervisor
> GI Pathology of Dayton
> Dig= estive Specialists, Inc
> Phone: (937) 396-2623
> Email: = [65]lblazek@digestivespecia= lists.com
>
>
>
> -----Original Mes= sage-----
> From: [66]histonet-boun...@lists.utsouthwestern.edu [mailto:
&= gt; [67]histonet-b= oun...@lists.utsouthwestern.edu] On Behalf
Of Connolly, Brett M
&= gt; Sent: Tuesday, April 30, 2013 9:54 AM
> To: k. leigh adams; [68]histonet@lists.utsouthwe= stern.edu
> Subject: RE: [Histonet] HIER pressure cookers....<= br>
>
> Biocare Decloaker here !!
>
> Brett= M. Connolly, Ph.D.
> Principal Scientist, Imaging Dept.
>= ; Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, = PA 19486
> [69]brett_conno= l...@merck.com
> T- 215-652-2501
> F- 215-993-6803
=
>
>
>
>
>
>
> -= ----Original Message-----
> From: [70]histonet-boun...@lists.utsouthwestern.edu = [mailto:
> [71]histonet-boun...@lists.utsouthwestern.edu] On Behalf Of k.
leigh = adams
> Sent: Tuesday, April 30, 2013 9:30 AM
> To: histonet@lists.utsouthweste= rn.edu
> Subject: [Histonet] HIER pressure cookers....
&= gt;
> Any input as to preferred instruments would be greatly appre ciated...
>
> Leigh
> __________________________= _____________________
> Histonet mailing list
> [72]Histonet@lists.utsouthwestern= .edu
> [73]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Notice: This e-mail message, together with any attachments, c ontains
> information of Merck & Co., Inc. (One Merck Drive, W= hitehouse
Station, New
> Jersey, USA 08889), and/or its affiliates= Direct contact
information for
> affiliates is available at
=
> [74]http://www.m= erck.com/contact/contacts.html) that may be
confidential,
> pr= oprietary copyrighted and/or legally privileged. It is
intended solely
=
> for the use of the individual or entity named on this message.
If y= ou are
> not the intended recipient, and have received this messag= e in
error, please
> notify us immediately by reply e-mail and the= n delete it from
your system.
>
>
> ____________= ___________________________________
> Histonet mailing list
= > [75]histo...@lists.ut= southwestern.edu
> [76]http://lists.utsouthwestern.edu/mailman/listinfo= /histonet
>
> _______________________________________= ________
> Histonet mailing list
> [77]Histonet@lists.utsouthwestern.edu
= > [78]= http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Jan Shivers
Senior Scientist
=
IHC/Histology Section Head
Pathology Teaching Program
UMN Ve= terinary Diagnostic Laboratory
University of Minnesota College of Vet= erinary Medicine
1333 Gortner Ave.
St. Paul, MN 55108
61= 2-624-7297
[79]shive...@umn.edu
------------------------------
Message: 8
Date: Tue, 30 Apr 2013 10:36:24 -0400
From: "Hannen, Valerie" &l= t;[80]Valerie.Hannen@parrishme= d.com>
Subject: RE: [Histonet] 5 gal specimen container search
To: 'Jackie O'Connor' <[81]b427297= @aol.com>,
"[82]histonet@lists.utsouthwestern.edu"
<[83]histonet@lists.utsouthwestern.edu&g= t;
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexsto re03>
Content-Type: text/plain; charset="us-ascii"
= Jackie...
We have ordered these from a company by the name of = Centurian
Medical. The lids are sold separately.
Valerie
=
-----Original Message-----
From: [84]histonet-boun...@lists.utsouthwestern.ed= u [[85]mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie
O'Connor
Sent: Wednesday, April 24, 2013 3:41 PM
To: [86]histonet@lists.utsouthwestern.edu
Subject: [Histonet] 5 gal specimen container search
=
Anyone in histoland know a vendor where I can find a 5 gallon
leakproof = bucket, drum, pail for shipping a large pathology
specimen?
Ja= ckie O'
_______________________________________________
= Histonet mailing list
[87]Histonet@lists.utsouthwestern.edu
[88]http://lists.utsouthwestern.ed= u/mailman/listinfo/histonet
=========== =========
"This email is intended solely for the us= e of the individual to
whom it is addressed and may contain informati= on that is
privileged, confidential or otherwise exempt from disclosu= re
under applicable law. If the reader of this email is not the
=
intended recipient or the employee or agent responsible for
delive= ring the message to the intended recipient, you are
hereby notified t= hat any dissemination, distribution, or
copying of this communication= is strictly prohibited. If you
have received this communication in e= rror, please immediately
delete this message. Thank you"
=== =================
------------------------------
Message: 9
Date: Tue,= 30 Apr 2013 10:38:59 -0400
From: "Shirley A. Powell" <[89]powell...@mercer.edu>
Subject: RE= : [Histonet] 5 gal specimen container search
To: "Hannen, Valerie" &l= t;[90]Valerie.Hannen@parrishme= d.com>,
'Jackie
O'Connor' <[91]b427...@aol.com>,
"[92]histonet@lists.utsouthwestern.edu"
<[93]histonet@lists.utsouthwestern.edu= >
Message-ID:
<9BF995BC0E47744E9673A41486E24EE2579
B14884C@[94]MERCERMAIL.MercerU.loca= l>
Content-Type: text/plain; charset="us-ascii"
=
You can also check with restaurant supply houses, we have
purchased grea= t large containers with lids for this purpose. Much
cheaper than the lab s= upply houses.
Shirley
-----Original Message-----<= br>
From: [95]hi stonet-boun...@lists.utsouthwestern.edu
[[96]mailto:histonet-bounces@lists.utsouthwestern= .edu] On Behalf Of
Hannen, Valerie
Sent: Tuesday, April 30, 2013 = 10:36 AM
To: 'Jackie O'Connor'; [97]histonet@lists.utsouthwestern.edu
Subject: RE: [H= istonet] 5 gal specimen container search
Jackie...
=
We have ordered these from a company by the name of Centurian
Medical. T= he lids are sold separately.
Valerie
-----Origin= al Message-----
From: [98]histonet-boun...@lists.utsouthwestern.edu
[[99]mailto:histonet-bounces@list= s.utsouthwestern.edu] On Behalf Of
Jackie O'Connor
Sent: Wednesda= y, April 24, 2013 3:41 PM
To: [100]histonet@lists.utsouthwestern.edu
Subject: [Histone= t] 5 gal specimen container search
Anyone in histoland = know a vendor where I can find a 5 gallon
leakproof bucket, drum, pail for = shipping a large pathology
specimen?
Jackie O'
__= _____________________________________________
Histonet mailing list
[101]histo...@lists.ut= southwestern.edu
[102]http://lists.utsouthwestern.edu/mailman/listinfo/hist= onet
=============
"This e= mail is intended solely for the use of the individual to
whom it is address= ed and may contain information that is privileged,
confidential or otherwis= e exempt from disclosure under applicable
law. If the reader of this email = is not the intended recipient or
the employee or agent responsible for deli= vering the message to the
intended recipient, you are hereby notified that = any dissemination,
distribution, or copying of this communication is strict= ly
prohibited. If you have received this communication in error, please
imm= ediately delete this message. Thank you"
========== ====
_______________________________________________
Histonet mailing
list
[103]Histonet@lists.utsouthwestern.edu
[104]http://lists.utsouthweste= rn.edu/mailman/listinfo/histonet
-----------= -------------------
Message: 10
Date: Tue, 30 Apr 2013 0= 7:40:51 -0700
From: <[105]dingers...@aplaboratories.com>
Subject: RE: [Histonet] HIE= R pressure cookers....
To: "Blazek, Linda" <[106]lbla...@digestivespecialists.com>, "Connolly,
Brett M" <[107]brett_conno...@merck.com>, "k. leigh adams"
<[108]k.leigh.adams....@gmail.com>= , "[109]histo...@lists.ut
southwestern.edu"
<[110]histonet@lists.utsouthwestern.edu>
Message-ID:
<[111]20130430074051.073ecbdb5144cf8a0
5e574ee22bfb11a.114d0c0283....@email17.secureserver.net>
Content-Type: text/plain; charset="utf-8"
[112]dingers...@aplaboratories.com
=
-------- Original Message --------
=
Subject: RE: [Histonet] HIER
From: "Blazek, Linda" <[1]lblazek@digestivespecialist= s.com>
To: "Connolly, Brett M" <[113]brett_conno...@merck.com>, " ada= ms"
<[2]k. "[3][114]histonet@lists.utsouthwestern.edu"
>&l= t;[4][115]histo...@lists.ut= southwestern.edu><
I second that!
=
Linda Blazek HT (ASCP)
GI = Pathology of Dayton
Digestive Speciali
Phone: (937= ) 396-2623
Email: [5][116]lbla...@digestivespecialists.com
-----Original Message-----
Fro= m: [6]histonet-bounces@[117]lists.utsouth= wes [118]mailto:h
istonet-boun...@lists.utsouthwestern.edu] On Behalf Of
= Connolly
Sent: Tuesday, April 30, 2013 9:54 AM
To= : k. leigh ad
Subject: RE: [Histonet] HIER pressure cookers... <= br>
Biocare Decloaker here !!
Brett M. Connolly, Ph
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
[9][119]brett_conno...@merck.com
T- 215-652-= 2501
F- 215-993-6803
=
----= -O
From: [10][120]histonet-boun...@lists.utsouthwestern.edu
=
[[11]mailto:histonet-bounc k. leigh adams
Sent: Tu
To: [12][121]histo= n...@lists.utsouthwestern.edu
Subject: [His
Any input as to preferred ins appreciated...
Leigh
_________
Histonet= mailing list
[13]Histonet@[122]= lists.utsouthwe
[14][123]http://lists.utsouthwestern.edu/mailman/listinf o/histonet
Notice: This e-mail message, together with a= ny attachments,
contai ns information of Merck & Co., Inc. (One= Merck Drive,
Whitehouse
Statio contact information
[15= ][124]http://www.merck.co= m/contact/contacts.html) that may
confidential, proprietary cop= yrighted and/or legally privileged. It
is
i this messag recei= ved this message in e-mail and then delete it
fro
=
________________________________________
Histo= net mailing list
[16][125]Histonet@lists.utsouthwestern.edu
[17]ht= tp://lists.uts
________________
Histonet= mailing list
[18]Histonet@[126]= lists.utsouthwestern
[19][127]http://lists.utsouthwestern.edu/mailman/li stinfo/histonet
References
1. 3D"mailto:lbla 2. 3D"[128]mailto:k.leigh.adams....@gmail.com";
3. 3D"mailto:h= istonet@[129]lists.utsout 4. file://localh
ost/tmp/3D"m 5. 3D"mailto:lblazek 6. 3D"mailto:h 7.
3D"[130]mailto:histonet-bounces@list= s.utsouthwestern.edu"
8. 3D"[131]mailto:histonet@lists.utsouthwestern.edu";
9= . 3D"mailto:bre 10. 3D"mailto:histonet-bounces@lists.u 11.
="[132]mailto:histonet-bounces@= lists.utsouthwestern.edu"
12. 3D"mailto:histo...@lists.ut 13. = 3D"[133]mailto:Histonet@lis
ts.utsouthwestern.edu"
14.
3D"[134]lists.utsouthwestern.edu/mailman/list">http://[135]lists.utsou
thwestern.edu/mailman/list 15.= 3D"[136]http://www.merck.com/conta 16.
3D"mailto:Histonet@lists. 17. file://localhost/tmp/3D" 18.
="mailto= :Histonet@lists.utsouthwestern.edu"
19. 3D"[137]lists.utsouthwestern.edu/mailman/listinfo">ht
tp://[138]lists.utsouthwestern.edu/mailman/l= istinfo/
------------------------------
Messa= ge: 11
Date: Tue, 30 Apr 2013 17:15:01 +0200
From: "Gudrun Lang= " ><[139]gu.l...@gmx.at>
Su= bject: AW: [Histonet] BIG PROBLEM
To: "'Behnaz Sohrab'" <[140]sohra...@ah.org>
Cc: [141]histonet@lists.utsouthwestern.edu
Message-ID: <000301ce45b5$7d292a50$777b7ef0$@[142]gmx.at>
Content-Type: text/plain; charset="iso-885= 9-1"
Too much blue is mostly a problem of too less red.
= Have you checked your Eosin? pH? Rinsing after eosin?
My perso= nal issue was sometimes too thin sections. Those looked
also rather
p= ale.
Gudrun
-----Urspr?ngliche Nachricht-----
=
Von: [143]histon= et-boun...@lists.utsouthwestern.edu
[[144]mailto:histonet-bounces@lists.utsouthweste= rn.edu] Im
Auftrag von Behnaz
Sohrab
Gesendet: Montag, 29. = April 2013 19:36
An: [145]histonet@lists.utsouthwestern.edu
Betreff: [Histonet] BIG PR= OBLEM
Good Morning, We have two Leica H&E stainer( old xt-= 5010 and new
5020). We
do use : Gill's 3 for 6 minutes, 4% Glacial AC= ETIC Acid 40
seconds Bluing :
45 sec. We done this for years!! last f= ew months our Pantologists
are
complaining about GI Biopsies that the= y have TOO much blue in
cytoplasma and
the Mucin is too purple and bl= ue? Please any suggestion??
Mean while we are trying the NEW kit from= Leica for H&E , they
think that is
TOO pale?any of you has tried= this new stain from Lecia?
With much application.
Behnaz Sohra= b, Los Angeles
------------------= ------------
Message: 12
Date: Tue, 30 Apr 2013 15:30:33= +0000
From: "Lake, Kim S" <[146]= kim-l...@uiowa.edu>
Subject: [Histonet] Processor question - a= lcoholic formalin vs.
buffered formalin
To: "[147]histonet@lists.utsouthwestern.edu"
<[148]histo= n...@lists.utsouthwestern.edu>
Message-ID:
<[149]f3181e1428ad274eaef67664c5b662ab25827...@itsnt443.iowa.uio
wa.edu><= br>
Content-Type: text/plain; charset="us-ascii"
I attende= d the Tri-State Symposium in Dubuque, IA last week
(which was fantastic) an= d sat in on a "Boot Camp for Histology"
workshop. At this workshop I reali= zed that some problems we have
been having with sectioning could be due to = our processor set up.
Before microtomy we have to soak our b= locks for 10-15 minutes,
and I suspect that this is because we are over deh= ydrating our
tissues in the during processing. Our first solution, which t= he
cassettes sit in for about 8 hours until the overnight processing
begins= , is alcoholic formalin, which is 450ml buffered formalin,
900ml water, and= 3300ml 95% alcohol. Our processor is a Leica TP 1050
that has been chuggi= ng along for about 20 years now.
The reason we use alcoholic f= ormalin instead of just normal
buffered formalin has been lost to the mists= of time, and I was
wondering what you all use as a holding solution on you= r processor,
and why.
Thanks!
Kim Lake
Labo= ratory Manager
Oral Pathology Laboratory
University of Iowa Col= lege of Dentistry
Phone (319) 384 4433
Fax (319) 353 5569
=
------------------------------
Message= : 13
Date: Tue, 30 Apr 2013 11:40:53 -0400
From: "Hannen, Valer= ie" <[150]Valerie.Hannen@par= rishmed.com>
Subject: RE: [Histonet] Processor question - alco= holic formalin
vs.
buffered formalin
To: "'Lake, Kim S'" &= lt;[151]kim-l...@uiowa.edu>,
= "[152]histo...@lists.uts= outhwestern.edu"
<[153]histonet@lists.utsouthwestern.edu>
Message-ID: < ;450B7A81EDA0C54E97C53D60F00776C3231B3D7C33@isexstore03>
Content-T= ype: text/plain; charset="us-ascii"
We use 10 % Neutral B= uffered Formalin. It is a wonderful
fixative and gives us really great fixa= tion.
It does sound like you are overly dehydrating your tissues.
=
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section C= hief
Parrish Medical Center
951 N. Washington Ave.
Titusv= ille, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 2= 68-6149
[154]valerie.hann= e...@parrishmed.com
-----Original Message-----
From: [155]h= istonet-boun...@lists.utsouthwestern.edu
[[156]mailto:histonet-bounces@lists.utsouthwester= n.edu] On Behalf Of
Lake, Kim S
Sent: Tuesday, April 30, 2013 11:= 31 AM
To: [157]histon= e...@lists.utsouthwestern.edu
Subject: [Histonet] Processor questio= n - alcoholic formalin vs.
buffered formalin
I attended the Tr= i-State Symposium in Dubuque, IA last week
(which was fantastic) and sat in= on a "Boot Camp for Histology"
workshop. At this workshop I realized that= some problems we have been
having with sectioning could be due to our proc= essor set up.
Before microtomy we have to soak our blocks fo= r 10-15 minutes,
and I suspect that this is because we are over dehydrating= our
tissues in the during processing. Our first solution, which the casse ttes
sit in for about 8 hours until the overnight processing begins,
is alc= oholic formalin, which is 450ml buffered formalin, 900ml
water, and 3300ml = 95% alcohol. Our processor is a Leica TP 1050 that
has been chugging along= for about 20 years now.
The reason we use alcoholic formalin = instead of just normal
buffered formalin has been lost to the mists of time= , and I was
wondering what you all use as a holding solution on your proces= sor,
and why.
Thanks!
Kim Lake
Laboratory M= anager
Oral Pathology Laboratory
University of Iowa College of = Dentistry
Phone (319) 384 4433
Fax (319) 353 5569
= _______________________________________________
Histonet mailing list
[158]Histonet@lists.= utsouthwestern.edu
[159]http://lists.utsouthwestern.edu/mailman/listinfo/hi= stonet
================== ==
"This email is intended solely for the use of the individual t= o
whom it is addressed and may contain information that is
priv= ileged, confidential or otherwise exempt from disclosure
under applic= able law. If the reader of this email is not the
intended recipient o= r the employee or agent responsible for
delivering the message to the= intended recipient, you are
hereby notified that any dissemination, = distribution, or
copying of this communication is strictly prohibited= . If you
have received this communication in error, please immediatel= y
delete this message. Thank you"
========== ==========
-----------------= -------------
Message: 14
Date: Tue, 30 Apr 2013 16:31:4= 9 +0000
From: "Margiotta-Watz, Michele" <[160]mmargio...@bmhmc.org>
Subject: [Histonet] casse= tte labelers
To: "'[161]histonet@lists.utsouthwestern.edu'"
<[162]histonet@lists.utsouthwestern.edu>
Message-ID:
<[163]230D0B9EC57D7A45A7A186C6AB4C7ABC
296f3...@bmh-exchange-02.bmhmc.org>
Content-Type: text/plain; = charset="us-ascii"
Hi All,
We are looking to purchase = 2 cassette labelers and would like
some feedback on which ones you might re= commend. Also, is there a
difference between Thermo's Printmate and Microwr= iter? Looks like
the Printmate is just a newer version. Your input would be= greatly
appreciated.
Thanks,
Michele
Histology Su= pervisor
BMHMC
631-654-7192
DISCLAIMER:
This= e-mail and any files transmitted with it are confidential and
are
in= tended solely for the use of the individual or entity to which
they
a= re addressed. This communication may contain material protected
by
t= he attorney-client privilege. If you are not the intended
recipient
= or the person responsible for delivering the e-mail to the
intended
r= ecipient, be advised that you have received this e-mail in error
and
= that any use, dissemination, forwarding, printing, or copying of
this
=
e-mail is strictly prohibited. If you have received this e-mail
in
=
error, please immediately notify the sender via return e-mail or
call
Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
------------------------------
___________________= ____________________________
Histonet mailing list
[164]Histonet@lists.utsouthwestern.edu<= /a>
[165]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 113, Issue 30
**********************= *******************
__________________________________________= _____
Histonet mailing list
[166]Histonet@lists.utsouthwestern.edu
[167]http://lists.utsou= thwestern.edu/mailman/listinfo/histonet
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file://localhost/tmp/3D"ht_______________________________________________
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