I do a Picro Sirius Red stain for collagen (got the protocol from Gayle Callis) and don't use Phosphomolybdic acid.
My protocol is simple: one hour in PSR two rinses in acidified water rinse you can counterstain but my clients don't want a counterstain so I dehydrate, clear and coverslip. Boom, I'm done. The PSR stain is just Sirius Red F3B 0.5 gms and 500 ml of Sat. Picric Acid. Do not use a dye that is not CI 35780. The acidified water is 5ml Glacial Acetic Acid to 1L of DH2O Under polarized light the PSR stain is gorgeous! I love looking at the orange, yellow, pinks and green colors. Good thing because I do this stain all the time. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu<mailto:algra...@email.arizona.edu> Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
