Dear John, I usually perform Picrosirius Red stain in cardiac tisse sections. I use phosphomolibic acid treatment in order to eliminate the cytoplasmic staining improving collagen/myoplasm contrast.
Have a look at this paper: Picrosirius red staining of cardiac muscle following phosphomolybdic acid treatment Dolber PC, Spach MS Stain Technol. 1987 Jan;62(1):23-6. Good luck! Laura > Hi All, > > Hope all are having a great Monday!!!! > > I am trying to do a Picro Sirius Red stain and have looked at many > protocols that seem to not use a particular reagent that is in a > manufacturers kit. I was hoping if someone could enlighten me as to why > this reagent would be used and if someone is using it at what strength is > the PhosoMolybdic Acid concentration. > > Your help will be greatly appreciated!!!! > > Kind Regards! > > John J Shelley > Research Specialist, Histology Core Facility > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Laura Avogaro University of Trento Via delle Regole, 101 38123 Mattarello (TN) Italy Tel: +39 0461 283425 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet