Thanks for the tip. I had some periodic acid so I tried a 2 minute soak in a 0.5% soln of that followed by a tap water wash and then dw then Harris hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical swine fever slides I looked at today. Very nice. I will play with this more.
By the way, We use the hot water/detergent method for all our dewaxing now. We still are using xylene and alcohols for our processing from fixative to paraffin but we will change that eventually. E. Wayne Johnson Enable Ag Tech Beijing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
