I have an ancient copy of Humason and she had methods 2 to 5 minutes for PAS etc with Periodic Acid so I tried 2 min today. Will try more time and stronger solutions and will also get around to doing some staining with PAS that I wanted to do to look at goblet cells and compare them to the vacuolated cells produced by porcine epidemic diarrhea...
> -------Original Message------- > From: Akemi Allison <akemiat3...@yahoo.com> > To: e...@pigsqq.org <e...@pigsqq.org>, histonet@lists.utsouthwestern.edu > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] periodic acid/h&e > Sent: Jul 05 '13 22:38 > > I forgot to mention the timing. You may try 5 minutes or even 10 minutes > before staining in hematoxylin. It will give you even better results. I used > 5 minutes in 1 % periodic acid. > > Akemi Allison BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3...@yahoo.com > > > > > ________________________________ > From: "e...@pigsqq.org" <e...@pigsqq.org> > To: histonet@lists.utsouthwestern.edu; """ > <histonet@lists.utsouthwestern.edu> > Sent: Friday, July 5, 2013 6:27 AM > Subject: [Histonet] periodic acid/h&e > > > Thanks for the tip. I had some periodic acid so I tried a 2 minute soak in > a 0.5% soln of that followed by a tap water wash and then dw then Harris > hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical > swine fever slides I looked at today. Very nice. I will play with this more. > > By the way, We use the hot water/detergent method for all our dewaxing now. > > We still are using xylene and alcohols for our processing from fixative to > paraffin but > we will change that eventually. > > E. Wayne Johnson > Enable Ag Tech > Beijing > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
