Jamie
We have sectioned these before and we used charged slides and did not have a
problem with section adherence. I would fix longer than 5 minutes. We would
use biopsy punches to remove the membrane from the well we would process the
disc whole between biopsy pads and bisect prior to embedding. You need to use
a good plus slide and it you are still having problems I would let the
unstained slides sit for a couple days prior to staining.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com
Ship to address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E
[jamie.erick...@abbvie.com]
Sent: Tuesday, July 09, 2013 2:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Section cells on a plastic trans well membrane
Hi All,
Has anyone tried to section cells cut from a trans well dish?
We have cells that are cultured and attach to a trans well membrane and our
pathologist wants to look at them on the membrane but the membrane is a thin
plastic film...see the problem.
We fixed the cells in the trans well for 5 minutes then cut the trans well out
of the well and bisected it and processed overnight we use paraffin type #1
paraffin. The next morning we embedded it with type #9 paraffin. The samples
cut but do not stay on the slide after xylene. I think the plastic does not
adhere to the slide. Would a adhesive slide work to keep the thin plastic stuck
to the slide??
My pathologist does not want to change to a collagen membrane so he wants me
to find another (harder paraffin) to use, I don't think that will help.
Has anyone does similar processing/embedding?
I'll take any help I can get..
Thanks,
Jamie
Abbvie Bioreseach Center
Pharmacology
100 Research Dr.
Worcester,Ma 01605
OFFICE +1 508-688-3134
FAX +1 508-793-4895
EMAIL jamie.erick...@abbvie.com<mailto:jamie.erick...@abbvie.com>
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