I used this method in a previous job which gave good results on 5um sections of FFPE mouse lung. The eosin counterstain allowed easier navigation around the tissue and meant the tissue was dark enough for the slide scanner to "see" automatically.
* Dewax and rehydrate to distilled water * Toluidine blue - 5 seconds * Running tap water - 20 seconds * 1% acetic acid - 1 second * Running tap water - 20 seconds * 0.5% Eosin - 1 second * Running tap water - 20 seconds * Ethanol - 45 seconds x3 * Xylene - 45 seconds x3 * Coverslip with DPX Andrew Prior Histologist Tissue Regenix Group E-mail: a.pr...@tissueregenix.com<mailto:a.pr...@tissueregenix.com> Website: www.tissueregenix.com<http://www.tissueregenix.com/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
