I have heard of and performed both methods. The variables seem to be with a 
ribbon, that if some fold, wrinkle, or fall off, you basically have more 
sections of the same depth on one slide. That being said, if that level is bad 
or  superficial, you will get recuts/deepers. If they are only needing one 
depth into the tissue for initial diagnosis or are worried about conserving 
tissue, then a ribbon might work. If they want progression, then you need more 
levels, one slide or many. Without going full rotations there wouldn't be much 
progression in depth in a single ribbon, especially at the thickness one would 
normally cut biopsy sections. Personally I like levels, one somewhat 
superficial section that is still a complete outline, one slightly deeper and 
one fully into the specimen without cutting anything away. Personally levels 
not only save recuts to me, but provide only one slide for labeling ( less 
labeling error opportunities), higher staining throughput/TAT/cases, less 
storage, less slides...you get the idea.  of course ultimately it is up to the 
Pathologist and sometimes others, but was there some reason given for the 
change in preference? 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: [email protected]
> To: [email protected]; [email protected]; 
> [email protected]
> Date: Tue, 23 Jul 2013 19:46:52 +0000
> CC: 
> Subject: [Histonet] RE: Sections
> 
> I have heard of both methods, currently we put double ribbons on one slide.
> The only time we have repeats is if the initial cut is superficial. 
> 
> -----Original Message-----
> From: [email protected] 
> [mailto:[email protected]] On Behalf Of Weems, Joyce 
> K.
> Sent: Tuesday, July 23, 2013 2:22 PM
> To: 'Martin, Gary'; [email protected]
> Subject: [Histonet] RE: Sections
> 
> If placing more than 1 section of the same level on a slide, I've always cut 
> three slides. I've never had a pathologist who only wanted to see one level.
> 
> Joyce Weems
> Pathology Manager
> 678-843-7376 Phone
> 678-843-7831 Fax
> [email protected]
> 
> 
> 
> www.saintjosephsatlanta.org
> 5665 Peachtree Dunwoody Road
> Atlanta, GA 30342
> 
> This e-mail, including any attachments is the property of Saint Joseph's 
> Hospital and is intended for the sole use of the intended recipient(s).  It 
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> -----Original Message-----
> From: [email protected] 
> [mailto:[email protected]] On Behalf Of Martin, Gary
> Sent: Tuesday, July 23, 2013 2:47 PM
> To: [email protected]
> Subject: [Histonet] Sections
> 
> One of our pathologist has requested a change in the way we cut GI specimens. 
>  We presently put three levels on one slide. Exp.  we face the block, cut a 
> ribbon and take one section of that ribbon and catch on the slide. We then 
> progress into the block and repeat the process until we have three levels on 
> the slide.  The new suggestion is to simply face the block, create a ribbon, 
> and take three to four section of that ribbon and that's it no more 
> progression into the block.  The thinking is that the Pathologist will see 
> what they need to in those levels, and if not they will call for "deepers". I 
> have never cut like this and just wanted to hear some thoughts on this method.
> 
> Thanks
> 
> Gary
> 
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