Agreed.
Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 23 Jul 2013 14:56:15 -0500 > From: [email protected] > To: [email protected] > Subject: Re: [Histonet] Sections > CC: [email protected]; [email protected] > > I've worked places where they've used all kinds of leveling methods, > specifying number of microns between levels, etc. I cut exactly whatever > the pathologist wants me to cut. I've traveled for 16 years now. Think > about some of the wacky crap I've seen. > A GOOD histotech, in my humble opinion, ( assuming a new, state of the > art mirotome, new disposable blade, cold, rough cut, hydrated blocks) > should be able to lay out a 21 section 3um ribbon of a GI bx longways on > the water bath in one go. > Said histotech would always ignore the first and last sections of > said 21 section ribbon (one is touching the waterbath, and the terminal one > touched the forceps/stick/finger/etc) and pick up the 2nd/3rd, 12th/13th, > and 19th/20th sections. This is 3 levels. > A BAD histotech will lay out a six section ribbon, take six sections > (and a floater), and call it 3 levels. > Make absolutely sure that you are rough cutting into the block > adequately to provide a full face section. Most of your recuts will come > from under-faced blocks. But try to cut and float nice LOOOOOOONG ribbons > to pick up your sections from and you will get nicer sections and less > recuts. What takes time cutting (traditional) levels is the refacing and > recooling/rehydration of the block. > If you can get true levels off of one long ribbon (not 6 adjacent > sections), and not increase recuts as a result, you win twice. So does the > patient. :) > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > > > > > > > > On Tue, Jul 23, 2013 at 2:04 PM, Tony Auge <[email protected]> wrote: > > > We cut the way your Pathologist is requesting at my lab, it's simpler and > > faster then the way you are currently cutting. I was used to cutting the > > way you described but this way is much easier. Check with your other > > Pathologists that they will be ok with the switch. I have certain > > Pathologist that will order more deepers because they want to see more of > > the block. It will speed up cutting in the first place but may take more > > time to cut deepers later in the day. > > -- > > > > Tony Auge HTL (ASCP) QIHC > > Cell: (651) 373-4768 > > Email: [email protected] > > _______________________________________________ > > Histonet mailing list > > [email protected] > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
