Dear colleagues, I am entertaining the notion of switching over to isopropyl alcohol (IPA) for our tissue processing and have a few questions. I know the xylene-free processing and microwave processing protocols use this universally, but at this time I am only interested in getting rid of the ethanol/methanol/isopropyl alcohol blend.
1 - Are there any known issues with holding tissues in 70% IPA rather than 70% ethanol/reagent alcohol? 2 - Is there any impact on the stain line if you use it for H&Es and hydration/dehydration of histochemically stained slides? 3 - Have any of you who use IPA found any impact on immunoreactivity when compared to using other dehydrants? 4 - Is there anything else I have overlooked? We have pure ethanol available to us if needed for particular stain applications. Best wishes, and thanks in advance. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
