The tissue processor can be cleaned with a strong glassware laboratory liquid soap at 5% in either hot water or hot IPA. The "hot" is the temperature provided by the tissue processor during the cleaning cycle. After you dry the stained sections in an oven at 60ºC for 5 minutes, you can place them directly in the coverslipper and they will be covered without any problems. René J.
________________________________ From: Brendal Finlay <brendal.fin...@medicalcenterclinic.com> To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 20, 2013 6:36 AM Subject: RE: [Histonet] Isopropyl Alcohol in the histology lab René, I'm very interested in your xylen free methods. I have a few questions for you, if you don't mine answering them. What is used on the clean cycle of your processor to remove paraffin waste? Our lab has a glass coverslipper. How would this work with coverslipping the slides manually or with our coverslipper? Thank you for all the information you offer to us at Histonet! Brendal Finlay, HT (ASCP) -----Original message----- From: Teri Johnson tjohn...@gnf.org Date: Tue, 13 Aug 2013 13:59:39 -0500 To: "Rathborne, Toni" trathbo...@somerset-healthcare.com, 'Rene J Buesa'rjbu...@yahoo.com, "'gu.l...@gmx.at'" gu.l...@gmx.at Subject: RE: [Histonet] Isopropyl Alcohol in the histology lab Teri: The automatic coverslipper will wok on oven dried stained sections. I used them on a Sakura film coverslipper and my lab was in Miami Beach, and you do not more humid than that! Xylene isthe one weakening the immunoreactivity the most but I have tested the IPA and the weakening does not exist although there are so many antibodies that some may weaken the issue is: will the weakening affect the diagnosis or just will produce a weaker reaction? That you would have to test further. But the bottom line is tha xylene should be eliminated. René J. Hi Rene, Have they changed the film coverslipper technology? When I used it years ago, you needed xylene to drop on to the slides to affix the film to them. The film coverslipper is not an option for us, we need the optical resolution that regular coverslips give for slide scanning. ~Teri _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet