Hi Gudrun, I hope you are well.
Another cause of anomalous staining is drying of the biopsy prior to fixative immersion. This can result in non-specific immunohistochemical staining as well as sub-optimal histochemical staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Saturday, 17 August 2013 7:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] edge artefact on core needle biopsy Hi all! We had a discussion on the cause of IHC positivity/false-positivity on the edges of core needle biopsies. (eg. breast, ER) One said, it is due to overfixation with formaldehyde-concentrations above 5%. (=false pos.) My opinion is, that's it's rather an effect of underfixation in the center in those cases. (= real pos.) In my lab, we rarely see this margin-effect and the biopsies are fixed for 6 hours at least. I searched in Pubmed for publications on this assay, but without success. Can anyone help me out with any literature? Or personal experience? Thanks in advance Gudrun Lang Ltd. BMA Histolab Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
