Donna S. Ing= ersoll, B.S., HTL, CT(ASCP) Laboratory Manager
A P Laboratories, LLC 2090 Executive Hall Rd S= uite 165 Charleston= , SC 29407 843-300-= 3001 X 202 843-300-= 3003 (fax) [1]dingers...@aplaboratories.com The contents of this message, together = with any attachments, are intended only for the use of the person(s) to whi= ch they are addressed and may contain confidential and/or privileged inform ation. Further, any medical information herein is confidential and protecte= d by law. It is unlawful for unauthorized persons to use, review, copy, dis= close, or disseminate confidential medical information. If you are not the = intended recipient, immediately advise the sender and delete this message a= nd any attachments. Any distribution, or copying of this message, or any at= tachment, is prohibited. -------- Original M= essage -------- Subject: Re: [Histonet] Xylene Substitutes From= : Rene J Buesa <[2]rjbu...@yahoo.com> Date: Wed, August 21, 2013 12:06 pm To: "Yaskovich, Ruth= A (NIH/NIDCR) [E]" <[3]rya skov...@dir.nidcr.nih.gov>, "'[4]Histonet@lists.utsouthwestern.edu'" <Histonet@lists.utsouthwest= ern.edu> Ruth: I all reality none is good. B= oth alkanes or D-Limonene are either as dangerous or more than xylene, and = all perform below the xylene standard. Any one requires modifications= of all procedures, they seldom dewax adequately and all constitute a proce= ssing "compromise". Many cannot de recycled. Please go to [5]http://www.histosearch.com/rene.html<= /a> and read about xylene substitution. The optimal solu= tion to eliminate xylene consists on: 1- dehydrate with 2-propanol (o= r "pure" isopropyl alcohol or IPA) 2- do not use any ante-medium and = go from IPA directly to paraffin wax or ideally to mineral oil:IPA at = 1:1 3- infiltrate as usual 4- dewax sections with 2%aq. sol. of= dishwater soap at 90ºC for 1 min twice 5- stain as usual = 6- dehydrate stained sections in a convection oven at 60ºC for 5 minut= es 7- coverslip as usual. As you can see xylene is nowhere to b= e found in this sequence that produce optimal quality slides. Try it! René J. ________________________________ From: "Yaskovich, Ruth A (NIH/NIDCR) [E]" <[6]ryaskov...@dir.nidcr.nih.gov> To: "'[7]Histonet@lists.utsouthwes= tern.edu'" <[8]His to...@lists.utsouthwestern.edu> Sent: Wednesday, August 21, 2= 013 11:18 AM Subject: [Histonet] Xylene Substitutes = I know this question has been asked before but what's the best and safes= t Xylene Substitute? Ruth Yaskovich N.I.H. N.I.D.C.R. ___= ____________________________________________ Histonet mailing list [9]histo...@lists.uts= outhwestern.edu [10]http://lists.utsouthwestern.edu/mailman/listinfo/histo= net _______________________________________________ Histone= t mailing list [11]H= isto...@lists.utsouthwestern.edu [12]http://lists.utsouthwestern.edu/mailm= an/listinfo/histonet References 1. ="mailto:dingers...@aplaboratories.com"; 2. 3D"mailto:rjbu...@yahoo.com"; 3. 3D"mailto:ryaskov...@dir.nidcr.nih.gov"; 4. 3D"mailto:Histonet@lists 5. file://localhost/tmp/3D" 6. 3D"mailto:ryaskov 7. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 8. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 9. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 10. 3D"http://lists.utsouthwestern.edu/mailma 11. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 12. 3D"http://lists.utsouthw=/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
