Hi, Would anyone suggest me what staining is best to color differentiate between cartilage and bone and epithelial tissues in avian embryos?
I have been trying Mallory Trichrome for embryos but recently I was suggested that Mallory Trichrome stains cartilage differently in embryos compared to adult samples since Aniline blue stains fiber that may not develop in early embryos. There is some protocol that modified the Mallory Trichrome that could be applied to embryos. However, the resulting colors of all tissues look all purple-ish and difficult to tell the cartilage from the weak blue stain from aniline blue. Currently I am thinking to try out Alcian blue/Hematoxylin and Eosin stain (Ehrlich’s hematoxylin). The purpose of the staining is to look at interaction between ossification and epithelial development so I think alcian blue for staining cartilage works but I am wondering if there is any other staining combination with alcian blue exist for visualizing bone and epithelial tissue (e,g. alcian blue/alizarine red with other staining?). Any suggestion would be appreciated! Rui TAHARA PhD Candidate Biology Department McGill University
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