At one time I did a lot of work on cartilage and growth plate
and used Toluidine Blue and Fast Green. T-blue (buffered appropriately)
stains the
proteoglycan a lovely metachromatic blue and the bone and most
everything else
is green. The nuclei of cells are also green since we used no nuclear
stain, but
that was not a problem for our work.
The cartilage is in stark contrast to the bone because of the high
carbohydrate
(glycosaminoglycan/proteoglycan) content of cartilage.
On 3:59, Rui TAHARA wrote:
Hi,
Would anyone suggest me what staining is
best to color differentiate between cartilage and bone and epithelial tissues
in avian embryos?
I have been trying Mallory Trichrome for
embryos but recently I was suggested that Mallory Trichrome stains cartilage
differently
in embryos compared to adult samples since Aniline blue stains fiber that may
not develop in early embryos. There is some protocol that modified the Mallory
Trichrome
that could be applied to embryos. However, the resulting colors of all tissues
look all purple-ish and difficult to tell the cartilage from the weak blue
stain from aniline blue.
Currently I am thinking to try out Alcian
blue/Hematoxylin and Eosin stain (Ehrlich’s hematoxylin). The purpose of the
staining is to look at interaction between ossification and epithelial
development
so I think alcian blue for staining cartilage works but I am wondering if there
is any other staining combination with alcian blue exist for visualizing bone
and epithelial tissue (e,g. alcian blue/alizarine red with other staining?).
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