However, I use the Shandon sequenza, and I still see edge effect. I always thought it was a fixation related. Maybe even that the edge staining is 'more real' than the rest!!
Histology, it's all voodoo!! Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Sep 24, 2013, at 7:51 AM, "Manfre, Philip" <[email protected]> wrote: > I concur with Rene'. That was my first thought. Edges drying up on an > autostainer due to hydrophobia or insufficient amounts of solution. Stainers > usually have the option to adjust the volume that is applied and sometimes > even the location on the slide (zones). Thorough deparaffinization is also > essential to avoid the hydrophobic repelling of solutions and wash buffers. > Also be sure your reagents have some form of surfactant or detergent > (e.g.Tween 20) in them to break the surface tension and allow the even spread > of whatever is being applied. > > Phil. > > > Philip Manfre, B.A., HT (ASCP) > Associate Principal Scientist > Merck Research Laboratories > WP45-251 > PO Box 4 > West Point, PA 19486 > > 215-652-9750 > 215-993-0383 (fax) > [email protected] > > > > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Rene J Buesa > Sent: Tuesday, September 24, 2013 10:36 AM > To: Mary Benoit; [email protected] > Subject: Re: [Histonet] edge effect on IHC > > Sometimes that "edge effect" is caused by drying when there is not enough > reagents. If you are using an automated stainer, please check the amount of > delivered reagents. > René J. > > > ________________________________ > From: Mary Benoit <[email protected]> > To: "[email protected]" <[email protected]> > Sent: Monday, September 23, 2013 5:00 PM > Subject: [Histonet] edge effect on IHC > > > I have been aware of some "edge effect" on an occasional slide, but lately we > noticed the effect is showing up in the area of the slide that is farthest > from the labels. We use DAKO Link Autostainer and their pretreatment baths > for everything. One case in particular we stained a large melanoma met to > colon that was well fixed and processed that stained only the edge, however > when we sent block to Neogenomics reference lab, their slide stained evenly > over the entire tumor area. We both used a melanoma cocktail. Not sure what > is going on. Any suggestions? > Thank you > Mary F Benoit, MT(ASCP) > The Pathology Laboratory > 830 Bayou Pines Drive > Lake Charles, LA 70601 > [email protected] > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
