Also make sure that the tissue never dries out on the trimming table before it even gets to formalin. That can also be a source of edge effect.
Jan Shivers UMN On Wed, Sep 25, 2013 at 7:37 AM, Tim Higgins <thiggin...@msn.com> wrote: > Hey Mary, > > > > I would suggest observing your > stainer to ensure the proper drop zones are being performed and not just > dropping closest to the label. I used Dako for years and loved them but > you do > have to make sure it dispenses properly from time to time. They use drop > zones > unlike Ventana which uses a vortex type of mixing. > > > > Good luck! > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > > > > > > Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT) > > From: Mary Benoit <mbp...@yahoo.com> > > Subject: [Histonet] edge effect on IHC > > To: "histonet@lists.utsouthwestern.edu" > > <histonet@lists.utsouthwestern.edu> > > Message-ID: > > <1379970057.43816.yahoomail...@web142505.mail.bf1.yahoo.com> > > Content-Type: text/plain; charset=us-ascii > > > > I have been aware of some "edge effect" on an occasional slide, but > lately we noticed the effect is showing up in the area of the slide that is > farthest from the labels. We use DAKO Link Autostainer and their > pretreatment baths for everything. One case in particular we stained a > large melanoma met to colon that was well fixed and processed that stained > only the edge, however when we sent block to Neogenomics reference lab, > their slide stained evenly over the entire tumor area. We both used a > melanoma cocktail. Not sure what is going on. Any suggestions? > > Thank you > > Mary F Benoit, MT(ASCP) > > The Pathology Laboratory > > 830 Bayou Pines Drive > > Lake Charles, LA 70601 > > mbp...@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet