We fix H&E's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 [email protected]
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee & Peggy Wenk; Stephenson, Sheryl; [email protected] Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: [email protected] [mailto:histonet- >[email protected]] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; [email protected] >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are >correct that "b" is a better answer. My students and I have found a >couple of other questions that we thought had the wrong answer >indicated in the study set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: [email protected] >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >[email protected] >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >[email protected] >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >[email protected] >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
