the protocol calls for 1 minute in the 40% formaldehyde and then rinse the sections well.
From: "Rathborne, Toni" <[email protected]> To: "'Jennifer MacDonald'" <[email protected]>, "Grantham, Andrea L - (algranth)" <[email protected]> Cc: HISTONET <[email protected]>, "[email protected]" <[email protected]> Date: 10/03/2013 11:42 AM Subject: RE: [Histonet] HT HistoDeck question... Sent by: [email protected] For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -----Original Message----- From: [email protected] [ mailto:[email protected]] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; [email protected] Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: "Grantham, Andrea L - (algranth)" <[email protected]> To: Cc: HISTONET <[email protected]> Date: 10/03/2013 08:33 AM Subject: Re: [Histonet] HT HistoDeck question... Sent by: [email protected] I'd go with "A", but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 [email protected]<mailto:[email protected]> Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
