When tissue processing was manual there were some "gadgets" providing vacuum and those using it reported better results. The fact of the matter was that manual processing is so slow that anything you introduce will favor the process. Static tissue processors, i.e. those that only mover the specimens circumventing the manual transfer like the HistoKinete only improved processing if they were able to move the specimens, something they did by adding rotation inside the reagents vessels. Retort tissue processors introduced 3 novelties: vacuum, pressure and, most importantly, agitation that is nothing but empty/fill the whole retort every 20 minutes. This agitation is more important, as you point out, than the vacuum/pressure. René J.
________________________________ From: Teri Johnson <[email protected]> To: "[email protected]" <[email protected]> Sent: Wednesday, October 2, 2013 6:57 PM Subject: [Histonet] Vacuum and pressure in tissue processing Dear friends, I recall hearing at a conference (or maybe it was just a casual conversation by an expert during a NSH symposium break) that vacuum and pressure in tissue processing really accomplishes very little. I do believe that using heat and agitation of the solutions provides more activity kinetically and therefore makes processing more efficient. Can someone affirm or deny the efficacy of vacuum and/or pressure in tissue processing, please? Thank you, as always, for your wisdom. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
