Fixation and processing has a huge effect on tissue size. My advice is to have them do the measurements prior to fixation upon harvest. That makes the fixation issue completely irrelevant. If you want to later validate it, you can control fixation time by using the same time (whatever you determine is appropriate) across multiple specimens and then store them in 70% ethanol to send. The important thing is to standardize everything you can.
Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
