You probably have water and/or alcohol contamination in your xylenes. Make sure your dehydrating alcohols after the eosin are really clean.
Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Coffey Sent: Monday, November 04, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with H&E Bleeding Hello Histonetters, We have recently noticed some bleeding of our H&E stained tissues and I was curious to hear if anyone has had problems with this before. There will just be a faint halo of stain around the tissue. This seems to be happening inconsistently (various tissues, even differences within the same stained batch) and has now occurred with both paraffin-embedded and frozen tissue, so with 2 different programs in our autostainer. If you've seen this in your lab before, were you able to isolate the cause and fix it? I'd be very appreciative of any advice you may have about what we can do to fix this problem. Thanks, Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ah...@georgetown.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
