ANP.23410 Cryostat Decontamination Phase II
There is a documented procedure for the routine decontamination of the cryostat 
at defined
intervals, and decontamination records are evident.
NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed 
surfaces with
tuberculocidal disinfectant. The cryostat should be at room temperature during 
decontamination
unless otherwise specified by the manufacturer. This should be done at an 
interval appropriate for
the institution; this must be weekly for instruments used daily. Trimmings and 
sections of tissue
that accumulate inside the cryostat must be removed during decontamination. 
Although not a
requirement, steel mesh gloves should be worn when changing knife blades.
REFERENCES
1) Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory 
Workers From Occupationally Acquired Infections; Approved
Guideline—Third Edition. CLSI document M29-A3 (ISBN 1-56238-567-4). Clinical 
and Laboratory Standards Institute, 940 West Valley
Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2005.
2) http://www.well.ox.ac.uk/_asset/file/leica-disinifection-2.pdf
3) http://www.epa.gov/oppad001/list_b_tuberculocide.pdf

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.
________________________________________
From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L 
[dorothy.l.w...@healthpartners.com]
Sent: Thursday, January 02, 2014 11:44 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] cryostat

Does anyone have any current information on the cryostats with UV for 
decontamination?  Specifically, is it accepted as a means of decontamination by 
CAP or JS or any other regulatory agencies?  Basically, is it worth the extra 
dollars to go that route?

Much thanks and Happy 2014!!

Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
651-254-2962



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