Tanya and Madeline, Jan Minshew of Leica sent a nicely detailed response to "Decontaminating" a cryostat in January of 2010. She apparently wrote it to put in a Leica "How To" manual, although I did not notice it in this form. Everything she states is well documented, although I also incorporate a known virucide and tuberculocidal product (following it's directions, naturally).
http://lists.utsouthwestern.edu/pipermail/histonet/2010-January/048750.html If you have a UV cryostat, use Leica's Cryofect product or follow Tim Morken's previous (2000 histosearch)formaldehyde method, they should work for killing most bugs too(with the exception of eradicating some prions like CJD/BSE). If I am misunderstanding what you mean by "Decon" I apologize, as there is no reason why you cannot clean up tissue debris in a cold cryostat with 100% alcohol. Hugh Hawaii ------------------------------ Date: Thu, 16 Jan 2014 10:43:09 -0800 (PST) From: Madeline Gi <madelin...@yahoo.com> Subject: Re: [Histonet] Cryostat To: "Abbott, Tanya" <tanyaabb...@catholichealth.net>, "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <1389897789.16602.yahoomail...@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I use to decon my cryostat with 100% Alco this keeps it from freezing up and it clean it. best of luck. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com On Wednesday, January 15, 2014 2:52 PM, "Abbott, Tanya" <tanyaabb...@catholichealth.net> wrote: Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
