Hi Jerry, Are you sure they are not AgNOR's:
Lindner, L. E. (1993). Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). Journal of Histochemistry & Cytochemistry, 41(3), 439-445. Thiebaut, F., Rigaut, J. P., & Reith, A. (1984). Improvement in the Specificity of the Silver Staining Technique for Ag NOR-ASSOCIATED Acidic Proteins in Paraffin Sections. Biotechnic & Histochemistry, 59(3), 181-185. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jerry sedgewick Sent: Friday, 24 January 2014 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver in Nucleolus I'm working with a lab that stained cat gonads with Warthin Starry. We're looking for Bartonella bacteria. In the first batch that was stained, some nucleii filled with the silver stain, something I've see in published images. In the second batch it appears that nucleolus filled with silver. I haven't seen that in google searches, etc. I wondered if others have seen the nucleolus fill with silver (or is this bartonella?). I'm also wondering how to prevent this sort of thing with proper bench procedures. Thanks! Jerry Sedgewick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
