Are you mixing Eosin in the last two alcohols of your processor? That should work. If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing.
Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink. I would do this under a dissecting microscope. Maybe you could do that with the eggs? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu <histonet-boun...@lists.utsouthwestern.edu> on behalf of Cheryl Crowder <ccrow...@vetmed.lsu.edu> Sent: Thursday, January 30, 2014 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet