Dedication to duty! Ouch! Did you ever run out of eyelashes? Why not a short
eyebrow or forearm hair?
JK
= = =
On 30/01/14, Barbara Tibbs <barbara.ti...@accuratediagnosticlabs.com> wrote:
> Are you mixing Eosin in the last two alcohols of your processor? That
> should work. If you are dotting the specimens with Eosin before placing in a
> cassette, the dye will wash out early in the processing.
>
> Also, I don't know if this helps you but when I had to ink mouse arteries I
> would glue one of my eyelashes to the end of a wood applicator stick and use
> the eyelash as a tiny paint brush dipped in India ink. I would do this under
> a dissecting microscope. Maybe you could do that with the eggs?
>
> Barbara S. Tibbs
> Histology Supervisor
> Accurate Diagnostic Labs
> South Plainfield, NJ
> barbara.ti...@accuratediagnosticlabs.com
> 732-839-3374
> Cell: 610-809-6508
>
>
> ________________________________________
> From: histonet-boun...@lists.utsouthwestern.edu
> <histonet-boun...@lists.utsouthwestern.edu> on behalf of Cheryl Crowder
> <ccrow...@vetmed.lsu.edu>
> Sent: Thursday, January 30, 2014 5:59 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] marking tiny specimens
>
> I am processing some extremely small specimens - pin tip size. These are
> eggs with a protein covering. I have tried using eosin to color the tissues
> before processing but the color came out before paraffin. The coating on
> the eggs will not absorb the dye. Does anyone have a suggestion for dyeing
> or marking these tissues so I can see them better to embed. Thanks in
> advance,
> Cheryl
>
> Cheryl Crowder, BA, HTL(ASCP)
>
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