Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). I have never stained with anything like this, so I ran a test.
I deparaffinized, blocked with NGS, incubated overnight at 4C with the diluted biotinylated SNA. On the second day, I used Vector's ABC kit and alkaline phosphatase (red) kit. Once stained, I noticed a lot of background. After looking into the blocking step, would a biotin/avidin blocking step be the correct step instead of a serum because I don't have a secondary? How do I know what needs to be blocked - biotin, avidin or both? Is there a way to do this without a kit and use solutions I may have in my lab? Thank you for your help! Sincerely, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
