What species are you looking to stain? Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiousis, Sam Sent: Wednesday, June 04, 2014 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microglia antibodies I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work using IHC and IF (regular, co-localization, even TSA amplification) on slides from frozen tissue that has been fixed in PFA and embedded in OCT with mixed crappy results. Does anyone have any suggestions or protocols? Thanks in advance Sam Kiousis Karmanos Cancer Institute skiou...@med.wayne.edu<mailto:skiou...@med.wayne.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
