I have used the CAP published 'Quality Management in Anatomic Pathology' and it has been very helpful with this:
"For a well-characterized antibody with a limited spectrum of antigenic targets, like chromogranin, or prostate specific antigen, the validation can be limited. A panel of 10 positive and 10 negative neoplasms would be sufficient in this setting. For an antibody that is not well characterized and/or has a wide range of reported activity, a more extensive validation is necessary. The number of tissues tested should in this circumstance be large enough to determine whether the staining profile matches that previously described. " We use cases known to be positive or negative for a given antibody or we request tumor types that are known to be positive or negative. We do 10 of each. If an antibody has a wide range of positivities (i.e. % positive like c-Myc or FOXP1), we try to have a range of different positivities for review. Hope this helps, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 [email protected]<mailto:[email protected]> This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
