Sheryl I will add another bit of information. That final antibody protein concentration is very low. Most of our antibodies (around 60 to 70%) will be in the range of 1 to 10 ug/ml. We do have some antibodies that can have some very low protein concentration though such as neurofilament.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
