Hi Liz, Wouldn't you dilute in 999.5ul of diluent, not 9999.5. 1ml=1000ul Otherwise I got the same answer... Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)213-2207
>>> Elizabeth Chlipala <l...@premierlab.com> 21/11/2014 3:35 PM >>> Sheryl Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same) V1 x C1 = V2 x C2 V1 - volume of stock solution needed - this is what you are solving for C1 - concentration of stock solution - you know this its 1.56 mg/ml or 1560 ug/ml (there are 1000 ug in one mg) V2 - volume of final solution needed - I put an arbitrary volume of 10 mls needed C2 - concentration of final solution - you know this its 0.8ug/ml V1 x 1560 ug/ml = 10ml x 0.8 ug/ml --- see how I made sure the units were the same I changed the mg/ml to ug/ml V1 = .0005 mls and then I would need 9.9995 mls of diluent to make up my 0.8ug/ml concentration, you can change this to be expressed in microliters by multiplying each number by 1000. So in uls - V1 = 0.5 uls and 9999.5 uls from there you can determine the titer which is your total volume divided by the volume of concentrated antibody 10/.0005 That would be a 1:20,000 dilution For a dilution such as this I would make up a stock solution of either 1:100 or 1:1000 and then dilute from there. Now its time for the rest of you to check my math, did I do this correctly????? I went over it a few times, but you never know.............. Have a GREAT Weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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