Hi all. Was just wondering if anyone has just taken the HTL exam. I passed the HT with just reading an older Frieda Carson book. Can someone give me some advice on books that really helped them? Thanks! Sent from my iPhone
> On Jan 23, 2015, at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: rodent eye (Gowan,Christie C) > 2. Cracking paraffin blocks (Wheelock, Timothy R.) > 3. RE: Cheap Disposable Blades for Facing In (Bea DeBrosse-Serra) > 4. Re: Cracking paraffin blocks (Hans B Snyder) > 5. Amyloid by Congo Red (Jeffrey Robinson) > 6. Thermo IHC (Cheri Miller) > 7. Problem with cracked paraffin blocks (Wheelock, Timothy R.) > 8. Re: Amyloid by Congo Red (Michael Ann Jones) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 21 Jan 2015 16:23:06 +0000 > From: "Gowan,Christie C" <christiecgo...@dermatology.med.ufl.edu> > Subject: RE: [Histonet] rodent eye > To: Casie Phillips <casie4...@gmail.com>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <ccc0568455733548a03568ac55691f76259...@ahc-mb02.ad.ufl.edu> > Content-Type: text/plain; charset="us-ascii" > > Hi Casie, > Hope by now you have rec'd some good tips on rodent eye prep. The only thing > I have to offer is that we always used Davidson's fixative for 24 hours and > then transferred to 70% ETOH. This worked beautifully preserving all eye > components. Good luck and don't forget to check the Histonet archives where I > know rodent eyes have been discussed in the past. > > Christie Gowan HT (ASCP) > > Department of Dermatology > 4037 NW 86th Terrace, 4th Fl > Mohs Laboratory > Gainesville, FL 32606 > Phone: 352 594-1529 > > > ________________________________________ > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] on behalf of Casie Phillips > [casie4...@gmail.com] > Sent: Thursday, January 15, 2015 2:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] rodent eye > > Good afternoon, > > I am currently working with Lewis rats performing corneal alkali injuries > at varying strengths. Is there someone there that has prior experience > working with a rat eye and would be willing to share information on the > most effective ways to preserve, fix and cut the cornea sample. > > We are interested in using the cornea without using the whole globe if > possible. For now we will be using basic H&E staining with a possibility of > immunohistochemistry at a later time. The main outcome we are looking for > is to find the presence of neutrophils in the cornea. A second objective is > to look for any damaged or newly reconstructed tissue. > > I would greatly appreciate any advice relating to the type of paraffin > used, the ideal length of time to save the tissue and any assistance you > can suggest for completing this process successfully. > > Thank you for your time. Any assistance will be greatly appreciated. > > Sincerely, > > Casie Phillips > casie4...@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Fri, 23 Jan 2015 14:57:15 +0000 > From: "Wheelock, Timothy R." <twheel...@mclean.harvard.edu> > Subject: [Histonet] Cracking paraffin blocks > To: "'Histonet@lists.utsouthwestern.edu'" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <69718c0b0b3c414d9f8e7214ad400cc9773ea...@phsx10mb11.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone: > > Recently, I purchased the Medite Valida Embedding Center, which I demoed > previously without a problem, if I recall right. > I am having problems now with blocks developing cracks on the cold plate. > The cracks run either through the wax right next to the specimen, or more > frequently, right through the tissue itself. > I use Surgipath Embedding Media (EM-400). > The surface of the Valida's cold plate is -14C. > > The company has not seen this happening before, but they are looking into > this further. > Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. > I do not think that this is a problem inherent with any particular machine. > Has anyone encountered this problem before? > If so, how did you resolve it? > > Is it possible that the -14C cold plate is too cold? Should I warm it up a > bit? > The surface of the Valida cold plate is, I believe, made out of smooth > stainless steel. > My old (24 years) Shandon Embedding Center's cold plate is made out of cast > aluminum and has a slightly "rougher" texture. > Could this produce a different way of conducting heat out of the paraffin > block than the Shandon? > Perhaps the stainless steel draws heat from the blocks faster than the > aluminum, and thus causes the cracking? > > I have used the Shandon Embedding Center for over 24 years. > I have never had a problem with blocks that crack. > > Thanks for any thoughts that you may have on this. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine > at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail. > > > ------------------------------ > > Message: 3 > Date: Fri, 23 Jan 2015 15:49:27 +0000 > From: Bea DeBrosse-Serra <bdebrosse-se...@isisph.com> > Subject: RE: [Histonet] Cheap Disposable Blades for Facing In > To: Jennifer MacDonald <jmacdon...@mtsac.edu>, Sandra Cheasty > <cheas...@svm.vetmed.wisc.edu> > Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <cb69cd3fbeb5524ca851930c971288fd329f1...@exch10mb01.isis.local> > Content-Type: text/plain; charset="us-ascii" > > We are doing the same. > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer > MacDonald > Sent: Friday, January 23, 2015 2:59 AM > To: Sandra Cheasty > Cc: Histonet (histonet@lists.utsouthwestern.edu) > Subject: Re: [Histonet] Cheap Disposable Blades for Facing In > > > We save our blade from the previous day to use for facing. We keep them in > slide mailers. > > Sent from my iPad > >>> On Jan 22, 2015, at 4:21 PM, Sandra Cheasty >> <cheas...@svm.vetmed.wisc.edu> wrote: >> >> Hello everyone, >> >> Does anyone have a source for cheap, low-profile >> blades > for facing in blocks? >> >> Thanks! >> Sandy >> >> >> >> Sandra J. Cheasty, HT (ASCP) >> >> Histology & Necropsy Supervisor, President Keith Richards Fan Club >> >> UW-Madison, School of Veterinary Medicine >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Fri, 23 Jan 2015 12:07:03 -0500 > From: Hans B Snyder <h...@histologistics.com> > Subject: Re: [Histonet] Cracking paraffin blocks > To: "Wheelock, Timothy R." <twheel...@mclean.harvard.edu> > Cc: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <CAAYBjcuB0-v=SwDCRjt5bpu=yegtudowm3_2atdsct4ijtp...@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Hello Tim, > > I also have had this cracking problem in the past but since resolved. > I'm not sure about the specifics of the cracking problem but the cold > plate might have something to do with it. Our cold plate is set to > -5C and use Paraplast from McCormick Scientific. Our paraffin is > roughly the same melting temp as yours, so probably not much > difference. > > Unfortunately trial and error is apart of our jobs since there is > relatively little uniformity in histology equipment and products. Have > you tried setting the cold plate to a warmer temperature yet? It's > worth a try and if that doesn't work maybe the paraffin has been > getting too hot. I know when the paraffin gets too hot, the wax and > plastic separate or break down and cause inconsistencies in the > paraffin. To test this, take an external thermometer and place inside > the paraffin tank. Then record the temp every hour for some time. > This will tell you if the tank itself is a consistent temperature. > > Also, are the blocks taken off the cold plate and immediately jarred > loose from the mold? Sometimes when I do this the shock of pried out > of the mold can cause the paraffin to become brittle and break rather > than bend. > > Let me know how it goes. > > Thank you > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > h...@histologistics.com > > > On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R. > <twheel...@mclean.harvard.edu> wrote: >> Hi Everyone: >> >> Recently, I purchased the Medite Valida Embedding Center, which I demoed >> previously without a problem, if I recall right. >> I am having problems now with blocks developing cracks on the cold plate. >> The cracks run either through the wax right next to the specimen, or more >> frequently, right through the tissue itself. >> I use Surgipath Embedding Media (EM-400). >> The surface of the Valida's cold plate is -14C. >> >> The company has not seen this happening before, but they are looking into >> this further. >> Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. >> I do not think that this is a problem inherent with any particular machine. >> Has anyone encountered this problem before? >> If so, how did you resolve it? >> >> Is it possible that the -14C cold plate is too cold? Should I warm it up a >> bit? >> The surface of the Valida cold plate is, I believe, made out of smooth >> stainless steel. >> My old (24 years) Shandon Embedding Center's cold plate is made out of cast >> aluminum and has a slightly "rougher" texture. >> Could this produce a different way of conducting heat out of the paraffin >> block than the Shandon? >> Perhaps the stainless steel draws heat from the blocks faster than the >> aluminum, and thus causes the cracking? >> >> I have used the Shandon Embedding Center for over 24 years. >> I have never had a problem with blocks that crack. >> >> Thanks for any thoughts that you may have on this. >> >> Tim Wheelock >> Harvard Brain Bank >> McLean Hospital >> Belmont, MA >> >> >> The information in this e-mail is intended only for the person to whom it is >> addressed. If you believe this e-mail was sent to you in error and the e-mail >> contains patient information, please contact the Partners Compliance >> HelpLine at >> http://www.partners.org/complianceline . If the e-mail was sent to you in >> error >> but does not contain patient information, please contact the sender and >> properly >> dispose of the e-mail. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Fri, 23 Jan 2015 17:43:33 +0000 > From: Jeffrey Robinson <jrobin...@pathology-associates.com> > Subject: [Histonet] Amyloid by Congo Red > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > > <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> > > Content-Type: text/plain; charset="us-ascii" > > Greetings to all Histotechs- Here's an amyloid question for the braintrust. > We are cutting our slides and controls at 9 and staining in Congo Red for 1 > hour. The control stains fine but the patient tissue is staining negative > even on cases that the pathologist assures us should be positive for amyloid. > We are using the Leica APEX charged slides with control and patient tissue > on the same slide. Does anyone have any thoughts on why the patient tissue > is not staining? Thanks! > > Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, > Clovis, CA. > > > This email and attachments may contain PHI that is privileged and > confidential and is not intended for any unauthorized person. If you, the > reader, are not the intended recipient you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. Do not read the email but instead reply to the sender and destroy > the message and any attachments. Thank you. > > > ------------------------------ > > Message: 6 > Date: Fri, 23 Jan 2015 11:54:06 -0600 > From: Cheri Miller <cmil...@physlab.com> > Subject: [Histonet] Thermo IHC > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Cc: "histonet-boun...@lists.utsouthwestern.edu" > <histonet-boun...@lists.utsouthwestern.edu> > Message-ID: <E3C81A010935EA41B379AC765103F3BF4000D6C102@olsrv12> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters! I need some help. We just acquired a Thermo IHC system. Can > anyone help us cut through the *****88*888 and give me some helpful tips? We > have practical experience on the Ventana systems only. Has anyone been > successful at using only 1 HIER buffer? Any tips on how to shorten the > offline retrieval process? Currently we are at over 40 mins. Seems to me a ph > of 7.6 -7.8 should work for most all of our antibodies. Thanks, Cheri > > Cheryl A. Miller HT ASCP cm > Physicians Laboratory, P.C. > 4840 F St. > Omaha , NE. 68117 > 402 731 4145 ext. 532 > Cell 402 980 2537 > Fax 402 731 8653 > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > ------------------------------ > > Message: 7 > Date: Fri, 23 Jan 2015 17:55:12 +0000 > From: "Wheelock, Timothy R." <twheel...@mclean.harvard.edu> > Subject: [Histonet] Problem with cracked paraffin blocks > To: "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <69718c0b0b3c414d9f8e7214ad400cc9773ea...@phsx10mb11.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Hi again everyone: > > I want to thank you all for your advice. > The consensus is that I have my new embedding center's cold plate set way too > low at -14C, and that I should try raising it to around -5C to cure my > cracked block problem. > I will run some test blocks over the weekend, and then embed them at this new > temperature on Monday. > Otherwise my Valida embedding center is working very nicely (as did the > HistoStar when I demoed it). > The cassette holding tank can accommodate a large number of brain specimens > and the controls are very easy to use. > Thanks again for your advice. > Have a great weekend. > > Tim > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine > at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail. > > > ------------------------------ > > Message: 8 > Date: Fri, 23 Jan 2015 17:58:31 +0000 > From: Michael Ann Jones <mjo...@metropath.com> > Subject: Re: [Histonet] Amyloid by Congo Red > To: Jeffrey Robinson <jrobin...@pathology-associates.com>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: <d0e7d992.65c3%mjo...@metropath.com> > Content-Type: text/plain; charset="us-ascii" > > What protocol and reagents are you using for the stain? > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > mjo...@metropath.com > > > > > > > On 1/23/15, 10:43 AM, "Jeffrey Robinson" > <jrobin...@pathology-associates.com> wrote: > >> Greetings to all Histotechs- Here's an amyloid question for the >> braintrust. We are cutting our slides and controls at 9 and staining in >> Congo Red for 1 hour. The control stains fine but the patient tissue is >> staining negative even on cases that the pathologist assures us should be >> positive for amyloid. We are using the Leica APEX charged slides with >> control and patient tissue on the same slide. Does anyone have any >> thoughts on why the patient tissue is not staining? Thanks! >> >> Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, >> Clovis, CA. >> >> >> This email and attachments may contain PHI that is privileged and >> confidential and is not intended for any unauthorized person. If you, the >> reader, are not the intended recipient you are hereby notified that any >> dissemination, distribution or copying of this communication is strictly >> prohibited. Do not read the email but instead reply to the sender and >> destroy the message and any attachments. Thank you. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 134, Issue 28 > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
