Agree with your points and book recommendations. I do remember some slide ID on
mine. Also there are more taxonomy II & III and operations on the HTL, which
warrants a slightly different study approach, but completely acheivable.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: jrobin...@pathology-associates.com
> To: scby...@yahoo.com; histonet@lists.utsouthwestern.edu
> Date: Fri, 23 Jan 2015 19:08:47 +0000
> Subject: RE: [Histonet] HTL exam
> CC:
>
> I used the NSH booklets on the various Histology subjects. I don't know
> about their current availability- I think they were on a CD now but I haven't
> checked lately. I learned a lot from the booklets as they not only give the
> correct answer they also described why the other answers were wrong along
> with some background pertaining to related subjects. With the test being
> online now I don't know how important the color plates of the various special
> stains are but I found it extremely helpful to know all of the stains by
> sight backwards and forwards- even stains that we did not run in our lab as
> there were a lot of questions that would refer to different methods of
> staining for the same structure or organism, etc. I used Sheehan and
> Bancroft as my texts. Bancroft is British so there is a different slant to
> his writing that I find interesting. I have read Carson's but I do not feel
> it has enough background information. Lee Luna's last book has great color
> plates but the organization is poor and it can be hard to find things- I
> think someone finished it up after he passed away.
>
> Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab,
> Clovis, CA.
>
> -----Original Message-----
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann
> Morissette
> Sent: Friday, January 23, 2015 10:43 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] HTL exam
>
>
> Hi all. Was just wondering if anyone has just taken the HTL exam. I passed
> the HT with just reading an older Frieda Carson book. Can someone give me
> some advice on books that really helped them? Thanks!
> Sent from my iPhone
>
> > On Jan 23, 2015, at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu
> > wrote:
> >
> > Send Histonet mailing list submissions to
> > histonet@lists.utsouthwestern.edu
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > or, via email, send a message with subject or body 'help' to
> > histonet-requ...@lists.utsouthwestern.edu
> >
> > You can reach the person managing the list at
> > histonet-ow...@lists.utsouthwestern.edu
> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Histonet digest..."
> >
> >
> > Today's Topics:
> >
> > 1. RE: rodent eye (Gowan,Christie C)
> > 2. Cracking paraffin blocks (Wheelock, Timothy R.)
> > 3. RE: Cheap Disposable Blades for Facing In (Bea DeBrosse-Serra)
> > 4. Re: Cracking paraffin blocks (Hans B Snyder)
> > 5. Amyloid by Congo Red (Jeffrey Robinson)
> > 6. Thermo IHC (Cheri Miller)
> > 7. Problem with cracked paraffin blocks (Wheelock, Timothy R.)
> > 8. Re: Amyloid by Congo Red (Michael Ann Jones)
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Wed, 21 Jan 2015 16:23:06 +0000
> > From: "Gowan,Christie C" <christiecgo...@dermatology.med.ufl.edu>
> > Subject: RE: [Histonet] rodent eye
> > To: Casie Phillips <casie4...@gmail.com>,
> > "histonet@lists.utsouthwestern.edu"
> > <histonet@lists.utsouthwestern.edu>
> > Message-ID:
> > <ccc0568455733548a03568ac55691f76259...@ahc-mb02.ad.ufl.edu>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Hi Casie,
> > Hope by now you have rec'd some good tips on rodent eye prep. The only
> > thing I have to offer is that we always used Davidson's fixative for 24
> > hours and then transferred to 70% ETOH. This worked beautifully preserving
> > all eye components. Good luck and don't forget to check the Histonet
> > archives where I know rodent eyes have been discussed in the past.
> >
> > Christie Gowan HT (ASCP)
> >
> > Department of Dermatology
> > 4037 NW 86th Terrace, 4th Fl
> > Mohs Laboratory
> > Gainesville, FL 32606
> > Phone: 352 594-1529
> >
> >
> > ________________________________________
> > From: histonet-boun...@lists.utsouthwestern.edu
> > [histonet-boun...@lists.utsouthwestern.edu] on behalf of Casie
> > Phillips [casie4...@gmail.com]
> > Sent: Thursday, January 15, 2015 2:53 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] rodent eye
> >
> > Good afternoon,
> >
> > I am currently working with Lewis rats performing corneal alkali
> > injuries at varying strengths. Is there someone there that has prior
> > experience working with a rat eye and would be willing to share
> > information on the most effective ways to preserve, fix and cut the cornea
> > sample.
> >
> > We are interested in using the cornea without using the whole globe if
> > possible. For now we will be using basic H&E staining with a
> > possibility of immunohistochemistry at a later time. The main outcome
> > we are looking for is to find the presence of neutrophils in the
> > cornea. A second objective is to look for any damaged or newly
> > reconstructed tissue.
> >
> > I would greatly appreciate any advice relating to the type of paraffin
> > used, the ideal length of time to save the tissue and any assistance
> > you can suggest for completing this process successfully.
> >
> > Thank you for your time. Any assistance will be greatly appreciated.
> >
> > Sincerely,
> >
> > Casie Phillips
> > casie4...@gmail.com
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Fri, 23 Jan 2015 14:57:15 +0000
> > From: "Wheelock, Timothy R." <twheel...@mclean.harvard.edu>
> > Subject: [Histonet] Cracking paraffin blocks
> > To: "'Histonet@lists.utsouthwestern.edu'"
> > <Histonet@lists.utsouthwestern.edu>
> > Message-ID:
> > <69718c0b0b3c414d9f8e7214ad400cc9773ea...@phsx10mb11.partners.org>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Hi Everyone:
> >
> > Recently, I purchased the Medite Valida Embedding Center, which I demoed
> > previously without a problem, if I recall right.
> > I am having problems now with blocks developing cracks on the cold plate.
> > The cracks run either through the wax right next to the specimen, or more
> > frequently, right through the tissue itself.
> > I use Surgipath Embedding Media (EM-400).
> > The surface of the Valida's cold plate is -14C.
> >
> > The company has not seen this happening before, but they are looking into
> > this further.
> > Also, I had the same problem when I demoed the Thermo-Fisher HistoStar.
> > I do not think that this is a problem inherent with any particular machine.
> > Has anyone encountered this problem before?
> > If so, how did you resolve it?
> >
> > Is it possible that the -14C cold plate is too cold? Should I warm it up a
> > bit?
> > The surface of the Valida cold plate is, I believe, made out of smooth
> > stainless steel.
> > My old (24 years) Shandon Embedding Center's cold plate is made out of
> > cast aluminum and has a slightly "rougher" texture.
> > Could this produce a different way of conducting heat out of the paraffin
> > block than the Shandon?
> > Perhaps the stainless steel draws heat from the blocks faster than the
> > aluminum, and thus causes the cracking?
> >
> > I have used the Shandon Embedding Center for over 24 years.
> > I have never had a problem with blocks that crack.
> >
> > Thanks for any thoughts that you may have on this.
> >
> > Tim Wheelock
> > Harvard Brain Bank
> > McLean Hospital
> > Belmont, MA
> >
> >
> > The information in this e-mail is intended only for the person to whom
> > it is addressed. If you believe this e-mail was sent to you in error
> > and the e-mail contains patient information, please contact the
> > Partners Compliance HelpLine at http://www.partners.org/complianceline
> > . If the e-mail was sent to you in error but does not contain patient
> > information, please contact the sender and properly dispose of the e-mail.
> >
> >
> > ------------------------------
> >
> > Message: 3
> > Date: Fri, 23 Jan 2015 15:49:27 +0000
> > From: Bea DeBrosse-Serra <bdebrosse-se...@isisph.com>
> > Subject: RE: [Histonet] Cheap Disposable Blades for Facing In
> > To: Jennifer MacDonald <jmacdon...@mtsac.edu>, Sandra Cheasty
> > <cheas...@svm.vetmed.wisc.edu>
> > Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)"
> > <histonet@lists.utsouthwestern.edu>
> > Message-ID:
> > <cb69cd3fbeb5524ca851930c971288fd329f1...@exch10mb01.isis.local>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > We are doing the same.
> >
> > Beatrice DeBrosse-Serra HT(ASCP)QIHC
> > Isis Pharmaceuticals
> > Antisense Drug Discovery
> > 2855 Gazelle Ct.
> > Carlsbad, CA 92010
> > 760-603-2371
> >
> >
> >
> > -----Original Message-----
> > From: histonet-boun...@lists.utsouthwestern.edu
> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
> > Jennifer MacDonald
> > Sent: Friday, January 23, 2015 2:59 AM
> > To: Sandra Cheasty
> > Cc: Histonet (histonet@lists.utsouthwestern.edu)
> > Subject: Re: [Histonet] Cheap Disposable Blades for Facing In
> >
> >
> > We save our blade from the previous day to use for facing. We keep them in
> > slide mailers.
> >
> > Sent from my iPad
> >
> >>> On Jan 22, 2015, at 4:21 PM, Sandra Cheasty
> >> <cheas...@svm.vetmed.wisc.edu> wrote:
> >>
> >> Hello everyone,
> >>
> >> Does anyone have a source for cheap, low-profile
> >> blades
> > for facing in blocks?
> >>
> >> Thanks!
> >> Sandy
> >>
> >>
> >>
> >> Sandra J. Cheasty, HT (ASCP)
> >>
> >> Histology & Necropsy Supervisor, President Keith Richards Fan Club
> >>
> >> UW-Madison, School of Veterinary Medicine
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet@lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 4
> > Date: Fri, 23 Jan 2015 12:07:03 -0500
> > From: Hans B Snyder <h...@histologistics.com>
> > Subject: Re: [Histonet] Cracking paraffin blocks
> > To: "Wheelock, Timothy R." <twheel...@mclean.harvard.edu>
> > Cc: "Histonet@lists.utsouthwestern.edu"
> > <Histonet@lists.utsouthwestern.edu>
> > Message-ID:
> >
> > <CAAYBjcuB0-v=SwDCRjt5bpu=yegtudowm3_2atdsct4ijtp...@mail.gmail.com>
> > Content-Type: text/plain; charset=UTF-8
> >
> > Hello Tim,
> >
> > I also have had this cracking problem in the past but since resolved.
> > I'm not sure about the specifics of the cracking problem but the cold
> > plate might have something to do with it. Our cold plate is set to
> > -5C and use Paraplast from McCormick Scientific. Our paraffin is
> > roughly the same melting temp as yours, so probably not much
> > difference.
> >
> > Unfortunately trial and error is apart of our jobs since there is
> > relatively little uniformity in histology equipment and products. Have
> > you tried setting the cold plate to a warmer temperature yet? It's
> > worth a try and if that doesn't work maybe the paraffin has been
> > getting too hot. I know when the paraffin gets too hot, the wax and
> > plastic separate or break down and cause inconsistencies in the
> > paraffin. To test this, take an external thermometer and place inside
> > the paraffin tank. Then record the temp every hour for some time.
> > This will tell you if the tank itself is a consistent temperature.
> >
> > Also, are the blocks taken off the cold plate and immediately jarred
> > loose from the mold? Sometimes when I do this the shock of pried out
> > of the mold can cause the paraffin to become brittle and break rather
> > than bend.
> >
> > Let me know how it goes.
> >
> > Thank you
> > Hans B Snyder
> > Histologistics
> > 60 Prescott Street
> > Worcester, MA 01605
> > 508-308-7800
> > h...@histologistics.com
> >
> >
> > On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R.
> > <twheel...@mclean.harvard.edu> wrote:
> >> Hi Everyone:
> >>
> >> Recently, I purchased the Medite Valida Embedding Center, which I demoed
> >> previously without a problem, if I recall right.
> >> I am having problems now with blocks developing cracks on the cold plate.
> >> The cracks run either through the wax right next to the specimen, or more
> >> frequently, right through the tissue itself.
> >> I use Surgipath Embedding Media (EM-400).
> >> The surface of the Valida's cold plate is -14C.
> >>
> >> The company has not seen this happening before, but they are looking into
> >> this further.
> >> Also, I had the same problem when I demoed the Thermo-Fisher HistoStar.
> >> I do not think that this is a problem inherent with any particular machine.
> >> Has anyone encountered this problem before?
> >> If so, how did you resolve it?
> >>
> >> Is it possible that the -14C cold plate is too cold? Should I warm it up a
> >> bit?
> >> The surface of the Valida cold plate is, I believe, made out of smooth
> >> stainless steel.
> >> My old (24 years) Shandon Embedding Center's cold plate is made out of
> >> cast aluminum and has a slightly "rougher" texture.
> >> Could this produce a different way of conducting heat out of the paraffin
> >> block than the Shandon?
> >> Perhaps the stainless steel draws heat from the blocks faster than the
> >> aluminum, and thus causes the cracking?
> >>
> >> I have used the Shandon Embedding Center for over 24 years.
> >> I have never had a problem with blocks that crack.
> >>
> >> Thanks for any thoughts that you may have on this.
> >>
> >> Tim Wheelock
> >> Harvard Brain Bank
> >> McLean Hospital
> >> Belmont, MA
> >>
> >>
> >> The information in this e-mail is intended only for the person to
> >> whom it is addressed. If you believe this e-mail was sent to you in
> >> error and the e-mail contains patient information, please contact the
> >> Partners Compliance HelpLine at
> >> http://www.partners.org/complianceline . If the e-mail was sent to
> >> you in error but does not contain patient information, please contact the
> >> sender and properly dispose of the e-mail.
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet@lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 5
> > Date: Fri, 23 Jan 2015 17:43:33 +0000
> > From: Jeffrey Robinson <jrobin...@pathology-associates.com>
> > Subject: [Histonet] Amyloid by Congo Red
> > To: "histonet@lists.utsouthwestern.edu"
> > <histonet@lists.utsouthwestern.edu>
> > Message-ID:
> >
> > <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.
> > local>
> >
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Greetings to all Histotechs- Here's an amyloid question for the
> > braintrust. We are cutting our slides and controls at 9 and staining in
> > Congo Red for 1 hour. The control stains fine but the patient tissue is
> > staining negative even on cases that the pathologist assures us should be
> > positive for amyloid. We are using the Leica APEX charged slides with
> > control and patient tissue on the same slide. Does anyone have any
> > thoughts on why the patient tissue is not staining? Thanks!
> >
> > Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab,
> > Clovis, CA.
> >
> >
> > This email and attachments may contain PHI that is privileged and
> > confidential and is not intended for any unauthorized person. If you, the
> > reader, are not the intended recipient you are hereby notified that any
> > dissemination, distribution or copying of this communication is strictly
> > prohibited. Do not read the email but instead reply to the sender and
> > destroy the message and any attachments. Thank you.
> >
> >
> > ------------------------------
> >
> > Message: 6
> > Date: Fri, 23 Jan 2015 11:54:06 -0600
> > From: Cheri Miller <cmil...@physlab.com>
> > Subject: [Histonet] Thermo IHC
> > To: "histonet@lists.utsouthwestern.edu"
> > <histonet@lists.utsouthwestern.edu>
> > Cc: "histonet-boun...@lists.utsouthwestern.edu"
> > <histonet-boun...@lists.utsouthwestern.edu>
> > Message-ID: <E3C81A010935EA41B379AC765103F3BF4000D6C102@olsrv12>
> > Content-Type: text/plain; charset="iso-8859-1"
> >
> > Hi Histonetters! I need some help. We just acquired a Thermo IHC
> > system. Can anyone help us cut through the *****88*888 and give me
> > some helpful tips? We have practical experience on the Ventana
> > systems only. Has anyone been successful at using only 1 HIER buffer?
> > Any tips on how to shorten the offline retrieval process? Currently we
> > are at over 40 mins. Seems to me a ph of 7.6 -7.8 should work for most
> > all of our antibodies. Thanks, Cheri
> >
> > Cheryl A. Miller HT ASCP cm
> > Physicians Laboratory, P.C.
> > 4840 F St.
> > Omaha , NE. 68117
> > 402 731 4145 ext. 532
> > Cell 402 980 2537
> > Fax 402 731 8653
> >
> > ________________________________
> > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If
> > you are not the addressee intended / indicated or agent responsible for
> > delivering it to the addressee, you are hereby notified that you are in
> > possession of confidential and privileged information. Any dissemination,
> > distribution, or copying of this e-mail is strictly prohibited. If you have
> > received this message in error, please notify the sender immediately and
> > delete this email from your system.
> >
> >
> > ------------------------------
> >
> > Message: 7
> > Date: Fri, 23 Jan 2015 17:55:12 +0000
> > From: "Wheelock, Timothy R." <twheel...@mclean.harvard.edu>
> > Subject: [Histonet] Problem with cracked paraffin blocks
> > To: "'histonet@lists.utsouthwestern.edu'"
> > <histonet@lists.utsouthwestern.edu>
> > Message-ID:
> > <69718c0b0b3c414d9f8e7214ad400cc9773ea...@phsx10mb11.partners.org>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Hi again everyone:
> >
> > I want to thank you all for your advice.
> > The consensus is that I have my new embedding center's cold plate set way
> > too low at -14C, and that I should try raising it to around -5C to cure my
> > cracked block problem.
> > I will run some test blocks over the weekend, and then embed them at this
> > new temperature on Monday.
> > Otherwise my Valida embedding center is working very nicely (as did the
> > HistoStar when I demoed it).
> > The cassette holding tank can accommodate a large number of brain specimens
> > and the controls are very easy to use.
> > Thanks again for your advice.
> > Have a great weekend.
> >
> > Tim
> >
> > Tim Wheelock
> > Harvard Brain Bank
> > McLean Hospital
> > Belmont, MA
> >
> >
> > The information in this e-mail is intended only for the person to whom
> > it is addressed. If you believe this e-mail was sent to you in error
> > and the e-mail contains patient information, please contact the
> > Partners Compliance HelpLine at http://www.partners.org/complianceline
> > . If the e-mail was sent to you in error but does not contain patient
> > information, please contact the sender and properly dispose of the e-mail.
> >
> >
> > ------------------------------
> >
> > Message: 8
> > Date: Fri, 23 Jan 2015 17:58:31 +0000
> > From: Michael Ann Jones <mjo...@metropath.com>
> > Subject: Re: [Histonet] Amyloid by Congo Red
> > To: Jeffrey Robinson <jrobin...@pathology-associates.com>,
> > "histonet@lists.utsouthwestern.edu"
> > <histonet@lists.utsouthwestern.edu>
> > Message-ID: <d0e7d992.65c3%mjo...@metropath.com>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > What protocol and reagents are you using for the stain?
> > Michael Ann Jones, HT (ASCP)
> > Histology Manager
> > Metropath
> > 7444 W. Alaska Dr. #250
> > Lakewood, CO 80226
> > 303.634.2511
> > mjo...@metropath.com
> >
> >
> >
> >
> >
> >
> > On 1/23/15, 10:43 AM, "Jeffrey Robinson"
> > <jrobin...@pathology-associates.com> wrote:
> >
> >> Greetings to all Histotechs- Here's an amyloid question for the
> >> braintrust. We are cutting our slides and controls at 9 and staining
> >> in Congo Red for 1 hour. The control stains fine but the patient
> >> tissue is staining negative even on cases that the pathologist
> >> assures us should be positive for amyloid. We are using the Leica
> >> APEX charged slides with control and patient tissue on the same
> >> slide. Does anyone have any thoughts on why the patient tissue is not
> >> staining? Thanks!
> >>
> >> Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology
> >> Lab, Clovis, CA.
> >>
> >>
> >> This email and attachments may contain PHI that is privileged and
> >> confidential and is not intended for any unauthorized person. If you,
> >> the reader, are not the intended recipient you are hereby notified
> >> that any dissemination, distribution or copying of this communication
> >> is strictly prohibited. Do not read the email but instead reply to
> >> the sender and destroy the message and any attachments. Thank you.
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet@lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> > ------------------------------
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > End of Histonet Digest, Vol 134, Issue 28
> > *****************************************
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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