I am afraid your tissue might be 'toast'. I tried to reprocess from that same issue and things did not go well. We were processing mouse tissue, which is dry enough, but then when we put the tissues back through xylene and into 100% and back again it got even worse.
We had to just get what we could from the blocks and confess to the researchers what had happened :( sorry for the bad news, I hope someone has better for you! mills On Thu, Feb 12, 2015 at 5:41 AM, Davenport, Martha R < martha.davenp...@uky.edu> wrote: > I am Martha Davenport, supervisor, at the University of KY histology lab. > We had a processing problem caused by accidently placing 70% (instead of > 100%) into the last dehydration container. Would anyone please give me info > on how you would remedy this? We usually reprocess the tissue but have had > trouble in the past with the tissue morphology being optimal. Any help will > be greatly appreciated. > Martha Davenport 859-257-1822 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet