Hi Martha- I have had some limited success in rehabilitating underprocessed tissue without reprocessing it in the tissue processor. If the tissue samples are not too large you may be able to let them air dry for a period of time so that they dry out. Make sure to face the blocks if you haven't already done so. You can then melt them down and put them in a mold with liquid paraffin and just let them sit on the embeddeder (still in liquid paraffin) for a couple of hours for better infiltration. Re-embed your samples and hope for the best. I hope this helps.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davenport, Martha R Sent: Thursday, February 12, 2015 5:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing problem I am Martha Davenport, supervisor, at the University of KY histology lab. We had a processing problem caused by accidently placing 70% (instead of 100%) into the last dehydration container. Would anyone please give me info on how you would remedy this? We usually reprocess the tissue but have had trouble in the past with the tissue morphology being optimal. Any help will be greatly appreciated. Martha Davenport 859-257-1822 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
