Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's, Dublin, Ireland
> On 9 Mar 2015, at 19:41, Bernice Frederick <b-freder...@northwestern.edu> > wrote: > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. > Sakura I would imagine. The issue here is that the coverslips have come up > from the slide and the tissue is adhered to the back of the coverslip. They > need to be recovered so they can be evaluated. What do you all recommend? We > use the CV5030 for coverslipping. I tried one with xylene and mounting media > but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
