To John
Probably the writer is referring to slides "mounted" with the Sakura film
"coverslip".I have done it many times and the FILM will detach easily from the
section.Had it been a glass coverslip attached with, for example, Permount,
acetone would not have done anything.René J.
On Tuesday, March 10, 2015 1:40 AM, John Kiernan <jkier...@uwo.ca> wrote:
Have you done this? Acetone does not dissolve resinous mounting media and
allow removal of coverslips. It's all in the books; buy one.
John Kiernan
Anatomy & Cell Biology, UWO
London, Canada
= = =
On 09/03/15, Jason McGough <jmcgo...@clinlab.com> wrote:
> Remove the film coverslip by placing the slide in acetone for a few minutes.
> Then recoverslip the slide with your current method.
>
>
>
> Jason McGough, HT(ASCP)
>
> Operations Manager
>
> Clinical Laboratory of the Black Hills
>
> 605-343-2267
>
> jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com <jmcgo...@clinlab.com>>
>
> www.clinlab.com <http://www.clinlab.com>
>
>
>
> -----Original message-----
> > From:Bernice Frederick <b-freder...@northwestern.edu
> > <mailto:b-freder...@northwestern.edu <b-freder...@northwestern.edu>> >
> > Sent: Monday, March 9, 2015 1:51 PM
> > To: histonet@lists.utsouthwestern.edu
> > <mailto:histonet@lists.utsouthwestern.edu
> > <histonet@lists.utsouthwestern.edu>>
> > Subject: [Histonet] Old slides.
> >
> > Hi all,
> > We received some old slides (1997-1998) that were coverslipped with film.
> > Sakura I would imagine. The issue here is that the coverslips have come up
> > from the slide and the tissue is adhered to the back of the coverslip. They
> > need to be recovered so they can be evaluated. What do you all recommend?
> > We use the CV5030 for coverslipping. I tried one with xylene and mounting
> > media but there were still a couple of air bubbles in there.
> > Thanks,
> > Bernice
> >
> > Bernice Frederick HTL (ASCP)
> > Senior Research Tech
> > Pathology Core Facility
> > Robert. H. Lurie Cancer Center
> > Northwestern University
> > 710 N Fairbanks Court
> > Olson 8-421
> > Chicago,IL 60611
> > 312-503-3723
> > b-freder...@northwestern.edu <mailto:b-freder...@northwestern.edu
> > <b-freder...@northwestern.edu>> <mailto:b-freder...@northwestern.edu
> > <b-freder...@northwestern.edu> <mailto:b-freder...@northwestern.edu
> > <b-freder...@northwestern.edu>> >
> >
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